使用玻璃体对相关死亡事故中的γ-羟丁酸(GHB)进行定量的意义:稳定性评估、病例报告和文献综述

IF 1.2 4区 医学 Q3 MEDICINE, LEGAL
Antoine Baudriller , Chadi Abbara , Marie Briet , Séverine Ferec , Léa-Héléna Rossi , Nathalie Jousset , Stéphane Malbranque , Guillaume Drevin
{"title":"使用玻璃体对相关死亡事故中的γ-羟丁酸(GHB)进行定量的意义:稳定性评估、病例报告和文献综述","authors":"Antoine Baudriller ,&nbsp;Chadi Abbara ,&nbsp;Marie Briet ,&nbsp;Séverine Ferec ,&nbsp;Léa-Héléna Rossi ,&nbsp;Nathalie Jousset ,&nbsp;Stéphane Malbranque ,&nbsp;Guillaume Drevin","doi":"10.1016/j.jflm.2024.102641","DOIUrl":null,"url":null,"abstract":"<div><p>Analysis and interpretation of the findings for γ-hydroxybutyrate (GHB) in related fatalities remains problematic. Indeed, GHB is a naturally occurring compound present in both the mammalian central nervous system and peripheral tissue. Moreover, a postmortem increase in endogenous GHB concentration has been observed, especially in blood. Facing this issue, the use of an alternative matrix such as vitreous humor (VH) can thus be particularly interesting for GHB testing and quantification. VH is considered to be less prone to postmortem redistribution, is easy to collect, and has relatively few interfering compounds for the analytical process. In this context, the authors report the case of a GHB-related fatality involving 22-year-old male. In this case, GHB femoral blood (FB) (790 mg/L) and vitreous (750 mg/L) concentrations appeared similar with a FB to VH (FB/VH) ratio of 1.05. In addition, other similar cases with both GHB blood and vitreous concentrations were reviewed. Five cases were identified. The blood to VH ratios ranging from 0.13 to 2.58. Finally, GHB stability was documented in postmortem blood and VH, in order to address the reliability of VH as an alternative matrix for GHB quantitation at postmortem. GHB appeared relatively stable in postmortem blood specimens (at 50 mg/L) over a period of 28 days when stored at +4 °C or −20 °C. The same results were observed in VH specimens.</p></div>","PeriodicalId":16098,"journal":{"name":"Journal of forensic and legal medicine","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1752928X24000039/pdfft?md5=dee01d5c939259ec1a5ace7a3d11b601&pid=1-s2.0-S1752928X24000039-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The interest of using vitreous humor for γ-hydroxybutyrate (GHB) quantification in related fatalities: Stability evaluation, case report and literature review\",\"authors\":\"Antoine Baudriller ,&nbsp;Chadi Abbara ,&nbsp;Marie Briet ,&nbsp;Séverine Ferec ,&nbsp;Léa-Héléna Rossi ,&nbsp;Nathalie Jousset ,&nbsp;Stéphane Malbranque ,&nbsp;Guillaume Drevin\",\"doi\":\"10.1016/j.jflm.2024.102641\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Analysis and interpretation of the findings for γ-hydroxybutyrate (GHB) in related fatalities remains problematic. Indeed, GHB is a naturally occurring compound present in both the mammalian central nervous system and peripheral tissue. Moreover, a postmortem increase in endogenous GHB concentration has been observed, especially in blood. Facing this issue, the use of an alternative matrix such as vitreous humor (VH) can thus be particularly interesting for GHB testing and quantification. VH is considered to be less prone to postmortem redistribution, is easy to collect, and has relatively few interfering compounds for the analytical process. In this context, the authors report the case of a GHB-related fatality involving 22-year-old male. In this case, GHB femoral blood (FB) (790 mg/L) and vitreous (750 mg/L) concentrations appeared similar with a FB to VH (FB/VH) ratio of 1.05. In addition, other similar cases with both GHB blood and vitreous concentrations were reviewed. Five cases were identified. The blood to VH ratios ranging from 0.13 to 2.58. Finally, GHB stability was documented in postmortem blood and VH, in order to address the reliability of VH as an alternative matrix for GHB quantitation at postmortem. GHB appeared relatively stable in postmortem blood specimens (at 50 mg/L) over a period of 28 days when stored at +4 °C or −20 °C. The same results were observed in VH specimens.</p></div>\",\"PeriodicalId\":16098,\"journal\":{\"name\":\"Journal of forensic and legal medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1752928X24000039/pdfft?md5=dee01d5c939259ec1a5ace7a3d11b601&pid=1-s2.0-S1752928X24000039-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of forensic and legal medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1752928X24000039\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of forensic and legal medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1752928X24000039","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0

摘要

对相关死亡事故中γ-羟丁酸(GHB)的分析和解释仍然存在问题。事实上,γ-羟丁酸是一种天然化合物,存在于哺乳动物的中枢神经系统和外周组织中。此外,已观察到死后内源性 GHB 浓度增加,尤其是在血液中。面对这一问题,使用玻璃体(VH)等替代基质进行 GHB 检测和定量就显得尤为重要。玻璃体液被认为不易在死后重新分布,易于收集,而且对分析过程的干扰化合物相对较少。在这种情况下,作者报告了一例与伽马--羟丁酸有关的死亡病例,死者是一名 22 岁的男性。在该病例中,伽马--羟丁酸(GHB)股血(FB)(790 毫克/升)和玻璃体(750 毫克/升)浓度相似,FB 与 VH(FB/VH)之比为 1.05。共发现了五个病例。血液与玻璃体的比率从 0.13 到 2.58 不等。最后,记录了死后血液和玻璃体中 GHB 的稳定性,以确定玻璃体作为死后 GHB 定量的替代基质的可靠性。在+4 °C或-20 °C条件下保存 28 天后,尸检血液样本(50 mg/L)中的伽马--羟丁酸显得相对稳定。在 VH 标本中也观察到了同样的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The interest of using vitreous humor for γ-hydroxybutyrate (GHB) quantification in related fatalities: Stability evaluation, case report and literature review

Analysis and interpretation of the findings for γ-hydroxybutyrate (GHB) in related fatalities remains problematic. Indeed, GHB is a naturally occurring compound present in both the mammalian central nervous system and peripheral tissue. Moreover, a postmortem increase in endogenous GHB concentration has been observed, especially in blood. Facing this issue, the use of an alternative matrix such as vitreous humor (VH) can thus be particularly interesting for GHB testing and quantification. VH is considered to be less prone to postmortem redistribution, is easy to collect, and has relatively few interfering compounds for the analytical process. In this context, the authors report the case of a GHB-related fatality involving 22-year-old male. In this case, GHB femoral blood (FB) (790 mg/L) and vitreous (750 mg/L) concentrations appeared similar with a FB to VH (FB/VH) ratio of 1.05. In addition, other similar cases with both GHB blood and vitreous concentrations were reviewed. Five cases were identified. The blood to VH ratios ranging from 0.13 to 2.58. Finally, GHB stability was documented in postmortem blood and VH, in order to address the reliability of VH as an alternative matrix for GHB quantitation at postmortem. GHB appeared relatively stable in postmortem blood specimens (at 50 mg/L) over a period of 28 days when stored at +4 °C or −20 °C. The same results were observed in VH specimens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.70
自引率
6.70%
发文量
106
审稿时长
57 days
期刊介绍: The Journal of Forensic and Legal Medicine publishes topical articles on aspects of forensic and legal medicine. Specifically the Journal supports research that explores the medical principles of care and forensic assessment of individuals, whether adult or child, in contact with the judicial system. It is a fully peer-review hybrid journal with a broad international perspective. The Journal accepts submissions of original research, review articles, and pertinent case studies, editorials, and commentaries in relevant areas of Forensic and Legal Medicine, Context of Practice, and Education and Training. The Journal adheres to strict publication ethical guidelines, and actively supports a culture of inclusive and representative publication.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信