使用简单的提取缓冲液和固相可逆固定（SPRI）纯化技术从微型磁带上的生物材料中提取 DNA

Q3 Medicine

Forensic Science International: Reports Pub Date : 2023-12-23 DOI:10.1016/j.fsir.2023.100350

Agnieszka Kuffel, Niamh Nic Daeid, Alexander Gray

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引用次数: 0

摘要

在这项研究中，我们比较了一种简单的 PVP 提取方法和一种市面上广泛使用的从胶带中提取 DNA 的试剂盒的性能。与 PrepFiler™ BTA 法医 DNA 提取试剂盒相比，这种新方法从 SceneSafe Fast™ 微型胶带上沉积的血液中提取 DNA 的回收率高出近 60%。我们还展示了如何通过对基于磁珠的纯化步骤进行简单修改来提高回收率和去除 PCR 抑制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification

In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast™ minitapes when compared to the PrepFiler™ BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.

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来源期刊

Forensic Science International: Reports Medicine-Pathology and Forensic Medicine

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

57 days