Feng Yan, Zaid A. Alhajeri, Adam Nyul-Toth, Chen Wang, Qinghao Zhang, Ebenezer Raj Selvaraj Mercyshalinie, Jordan Delfavero, Chetan Ahire, Bornface M. Mutembei, Stefano Tarantini, Anna Csiszar, Qinggong Tang
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引用次数: 0
摘要
脑微血管健康是研究自然衰老和相关神经疾病的关键生物标志物。我们的目的是量化小鼠大脑微血管在不同维度上与衰老相关的变化。我们使用光学相干断层扫描(OCT)和双光子显微镜(TPM)获得了非衰老和衰老 C57BL/6J 小鼠的活体脑微血管图像。结果表明,非老龄小鼠和老龄小鼠的动脉和静脉、动脉和静脉以及毛细血管在密度、直径、复杂程度、周长和迂曲程度上存在显著差异。OCT 血管造影和 TPM 弥补了每种模式的局限性,为动脉和静脉提供了全面的量化。我们进一步证明,在大多数量化分析中,非老年小鼠和老年小鼠的动脉和静脉在特定尺寸上呈现负相关,这表明 TPM 和 OCT 能够为研究衰老过程中脑血管的变化提供互补的血管信息。
Dimension-based quantification of aging-associated cerebral microvasculature determined by optical coherence tomography and two-photon microscopy
Cerebral microvascular health is a key biomarker for the study of natural aging and associated neurological diseases. Our aim is to quantify aging-associated change of microvasculature at diverse dimensions in mice brain. We used optical coherence tomography (OCT) and two-photon microscopy (TPM) to obtain nonaged and aged C57BL/6J mice cerebral microvascular images in vivo. Our results indicated that artery & vein, arteriole & venule, and capillary from nonaged and aged mice showed significant differences in density, diameter, complexity, perimeter, and tortuosity. OCT angiography and TPM provided the comprehensive quantification for arteriole and venule via compensating the limitation of each modality alone. We further demonstrated that arteriole and venule at specific dimensions exhibited negative correlations in most quantification analyses between nonaged and aged mice, which indicated that TPM and OCT were able to offer complementary vascular information to study the change of cerebral blood vessels in aging.
期刊介绍:
The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.