微生物电解池系统中进化的大肠杆菌 LGE2-H 提高了生物乙醇产量

IF 4.3 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cong Wang, Dongdong Chang, Qi Zhang, Zhisheng Yu
{"title":"微生物电解池系统中进化的大肠杆菌 LGE2-H 提高了生物乙醇产量","authors":"Cong Wang, Dongdong Chang, Qi Zhang, Zhisheng Yu","doi":"10.1186/s40643-023-00717-5","DOIUrl":null,"url":null,"abstract":"<p>Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved <i>Escherichia coli</i> to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the <i>E. coli</i>-H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, <i>E. coli</i>-H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":9067,"journal":{"name":"Bioresources and Bioprocessing","volume":"27 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced bioethanol production by evolved Escherichia coli LGE2-H in a microbial electrolysis cell system\",\"authors\":\"Cong Wang, Dongdong Chang, Qi Zhang, Zhisheng Yu\",\"doi\":\"10.1186/s40643-023-00717-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved <i>Escherichia coli</i> to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the <i>E. coli</i>-H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, <i>E. coli</i>-H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application.</p><h3 data-test=\\\"abstract-sub-heading\\\">Graphical abstract</h3>\",\"PeriodicalId\":9067,\"journal\":{\"name\":\"Bioresources and Bioprocessing\",\"volume\":\"27 1\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-01-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresources and Bioprocessing\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1186/s40643-023-00717-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresources and Bioprocessing","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s40643-023-00717-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

利用热解法预处理木质纤维素,可通过微生物发酵产生清洁能源(如生物乙醇),这对废物回收利用、环境保护和能源安全大有裨益。然而,生物油中的酸类、醛类和酚类物质对微生物有抑制作用,会影响木质纤维素热解物的下游利用和转化。在本研究中,我们利用进化大肠杆菌构建了一个微生物电解池系统,用于生物油解毒和高效乙醇生产,以克服以往研究中突出的生物乙醇生产和利用难题。在经电解处理的生物油培养基中,与对照组相比,大肠杆菌-H 菌株的左旋葡聚糖消耗量和乙醇生产能力显著提高。在含有 1.0%(w/v)左旋葡聚糖的未解毒生物油培养基中,大肠杆菌-H 产生了 0.54 克乙醇/克左旋葡聚糖,达到了理论产量的 94%。我们的研究结果将有助于开发一种利用木质纤维素基质生产生物乙醇的实用方法,并为其工业化应用提供科学依据和技术示范。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhanced bioethanol production by evolved Escherichia coli LGE2-H in a microbial electrolysis cell system

Enhanced bioethanol production by evolved Escherichia coli LGE2-H in a microbial electrolysis cell system

Lignocellulose pretreated using pyrolysis can yield clean energy (such as bioethanol) via microbial fermentation, which can significantly contribute to waste recycling, environmental protection, and energy security. However, the acids, aldehydes, and phenols present in bio-oil with inhibitory effects on microorganisms compromise the downstream utilization and conversion of lignocellulosic pyrolysates. In this study, we constructed a microbial electrolysis cell system for bio-oil detoxification and efficient ethanol production using evolved Escherichia coli to overcome the bioethanol production and utilization challenges highlighted in previous studies. In electrically treated bio-oil media, the E. coli-H strain exhibited significantly higher levoglucosan consumption and ethanol production capacities compared with the control. In undetoxified bio-oil media containing 1.0% (w/v) levoglucosan, E. coli-H produced 0.54 g ethanol/g levoglucosan, reaching 94% of the theoretical yield. Our findings will contribute to developing a practical method for bioethanol production from lignocellulosic substrates, and provide a scientific basis and technical demonstration for its industrialized application.

Graphical abstract

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bioresources and Bioprocessing
Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
8.70%
发文量
118
审稿时长
13 weeks
期刊介绍: Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信