器官组织协同工作:体外工程模型的保真度不断提高

IF 13.9 Q1 CHEMISTRY, MULTIDISCIPLINARY
Zhengkun Chen, Ryohichi Sugimura, Yu Shrike Zhang, Changshun Ruan, Chunyi Wen
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引用次数: 0

摘要

器官组织已成为体外研究复杂生物过程和疾病的强大平台。然而,大多数研究都集中在单个器官上,忽略了体内器官间的相互作用,限制了模型的生理相关性。为了解决这一局限性,多器官系统的开发受到了广泛关注。该系统旨在再现器官间的交流,从而研究复杂的生理过程。本综述全面概述了类器官工程学的最新进展以及构建多器官系统的新兴策略。首先,我们强调了设计适合不同类器官生长的材料所涉及的关键机械、结构和生化因素。此外,我们还讨论了如何结合动态培养环境来加强类器官培养并实现类器官之间的交流。此外,我们还探讨了操纵类器官形态发生和类器官空间定位的技术,以建立有效的类器官间通讯网络。我们总结了利用类器官再现体外器官间通讯的成就,包括组装体和微流体多类器官平台。最后,我们讨论了开发多器官系统的现有挑战和机遇,包括其可扩展性方面的技术瓶颈及其在复杂人类疾病方面的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Organoids in concert: engineering in vitro models toward enhanced fidelity

Organoids in concert: engineering in vitro models toward enhanced fidelity

Organoids in concert: engineering in vitro models toward enhanced fidelity

Organoids have emerged as a powerful platform for studying complex biological processes and diseases in vitro. However, most studies have focused on individual organoids, overlooking the inter-organ interactions in vivo and limiting the physiological relevance of the models. To address this limitation, the development of a multi-organoid system has gained considerable attention. This system aims to recapitulate inter-organ communication and enable the study of complex physiological processes. This review provides a comprehensive overview of the recent advancements in organoid engineering and the emerging strategies for constructing a multi-organoid system. First, we highlight the critical mechanical, structural, and biochemical factors involved in designing suitable materials for the growth of different organoids. Additionally, we discuss the incorporation of dynamic culture environments to enhance organoid culture and enable inter-organoid communication. Furthermore, we explore techniques for manipulating organoid morphogenesis and spatial positioning of organoids to establish effective inter-organoid communication networks. We summarize the achievements in utilizing organoids to recapitulate inter-organ communication in vitro, including assembloids and microfluidic multi-organoid platforms. Lastly, we discuss the existing challenges and opportunities in developing a multi-organoid system from its technical bottlenecks in scalability to its applications toward complex human diseases.

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CiteScore
17.40
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