海洋软体动物中长链多不饱和脂肪酸池的感知和调节:缢蛏中的 UBXD8 的特征

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhaoshou Ran , Haixuan Xie , Xuxu Tian , Fei Kong , Kai Liao , Xiaojun Yan , Jilin Xu
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引用次数: 0

摘要

缢蛏(Sinonovacula constricta)以富含长链多不饱和脂肪酸(LC-PUFA,C ≥ 20)而闻名。此前,我们已证明它拥有完整的 LC-PUFA 生物合成途径。然而,它感知 LC-PUFA 池以调节其生物合成的机制仍不清楚。在这里,我们介绍了低氯-PUFA 传感器 UBXD8,它是这一有趣过程中的一个关键分子。S. constricta UBXD8(ScUBXD8)具有哺乳动物对应物的所有特征,并在消化组织中表现出较高的 mRNA 水平,表明其在该双壳类动物中的功能作用。通过体外纯化 ScUBXD8 蛋白,我们发现它能感知不饱和脂肪酸(UFA,C ≥ 14),但不能感知饱和脂肪酸,聚合检测证明了这一点。此外,ScUBXD8 的聚合强度随酰基链长度、不饱和度和 UFA 浓度的增加而逐渐增加。与 n-3 UFA 相比,位于 LC-PUFA 生物合成途径同一节点的 ScUBXD8 对 n-6 UFA 的敏感性更高。这些结果表明,ScUBXD8 在平衡 S. constricta 中的脂肪酸组成和 n-6/n-3 UFA 比例方面起着关键作用。此外,UAS结构域被证实对ScUBXD8的聚合至关重要。通过在体内敲除 ScUbxd8 基因,与 LC-PUFA 生物合成相关的基因表达模式发生了显著变化,同时影响了脂肪酸组成。这些结果表明,ScUBXd8 可能通过 INSIG-SREBP 途径在 LC-PUFA 生物合成过程中发挥调控作用。综上所述,本研究认为缢蛏可能通过 UBXD8 来调节 LC-PUFA 的生物合成,从而维持 LC-PUFA 的平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sensing and regulation of long-chain polyunsaturated fatty acids pool in marine mollusks: Characterization of UBXD8 from the razor clam Sinonovacula constricta

The razor clam Sinonovacula constricta is known for its richness in long-chain polyunsaturated fatty acids (LC-PUFA, C ≥ 20). Previously, we demonstrated that it possesses a complete LC-PUFA biosynthetic pathway. However, the mechanisms by which it senses the LC-PUFA pool to regulate their biosynthesis remain unclear. Here, we presented the LC-PUFA sensor UBXD8 as a critical molecule in this intriguing process. The S. constricta UBXD8 (ScUBXD8) shared all characteristic features of its mammalian counterpart and exhibited high mRNA levels in digestive tissues, suggesting its functional role in this bivalve species. By purification of ScUBXD8 protein in vitro, we discovered its ability to sense unsaturated fatty acids (UFA, C ≥ 14) but not saturated ones, as evidenced by polymerization detection. Furthermore, the intensity of ScUBXD8 polymerization increased progressively with longer acyl chain lengths, greater unsaturation degrees, and higher UFA concentrations. Exceptionally, for those located at the same node in LC-PUFA biosynthetic pathway, ScUBXD8 displayed a stronger sensitivity to n-6 UFA compared to n-3 UFA. These results suggested a critical role for ScUBXD8 in balancing fatty acids composition and ratio of n-6/n-3 UFA in S. constricta. Moreover, the UAS domain was confirmed essential for ScUBXD8 polymerization. Through knockdown of ScUbxd8 gene in vivo, there were significant shifts in expression patterns of genes related to LC-PUFA biosynthesis, concurrently influencing fatty acids compositions. These results suggested that ScUBXD8 likely plays a regulatory role in LC-PUFA biosynthesis, possibly through the INSIG-SREBP pathway. Collectively, this study proposed that S. constricta might maintain LC-PUFA homeostasis through UBXD8 to regulate their biosynthesis.

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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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