通过非靶向脂质组学发现斯约格伦-拉尔森综合征的新型诊断生物标记物

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Frédéric M. Vaz , Pippa Staps , Jan Bert van Klinken , Henk van Lenthe , Martin Vervaart , Ronald J.A. Wanders , Mia L. Pras-Raves , Michel van Weeghel , Gajja S. Salomons , Sacha Ferdinandusse , Ron A. Wevers , Michèl A.A.P. Willemsen
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引用次数: 0

摘要

目的 薛格伦-拉森综合征(SLS)是一种罕见的神经代谢性疾病,主要影响大脑、眼睛和皮肤,由脂肪醛脱氢酶缺乏引起。我们最近发现,SLS 的脑组织脂质体严重紊乱,这促使我们寻找血浆中类似的生物标志物,因为目前还没有针对 SLS 的血液功能检测方法。方法与结果我们进行了血浆脂质组学研究,并使用新开发的生物信息学工具挖掘 SLS 血浆和脑脂质体的非靶向部分,以寻找 SLS 的生物标志物。血浆脂质组学显示,已知脂质类别的醚脂代谢紊乱。血浆和大脑(白质和灰质)的非靶向脂质组学发现了两种在 SLS 中高度升高的新的内源性脂质类别。第一组生物标志物是含有不同长度烷基链的烷基磷酸胆碱/乙醇胺,其中一些烷基磷酸胆碱在 SLS 血浆中升高了 600 倍。第二组生物标志物是一组结构未知的 5 个特征。碎片研究表明,它们含有泛醌醇和磷酸胆碱,其中一个特征在血浆中还发现了葡萄糖醛酸共轭物。血浆中的 SLS 特征非常明显,即使存在,其含量也是对照组的 100-1000 倍。我们推测了烷基磷酸胆碱/乙醇胺的来源以及含泛酸代谢物的性质。它们代表了首个血浆代谢物 SLS 生物标记物,也可能为 SLS 的病理生理学提供更多信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Discovery of novel diagnostic biomarkers for Sjögren-Larsson syndrome by untargeted lipidomics

Aim

Sjögren-Larsson syndrome (SLS) is a rare neurometabolic disorder that mainly affects brain, eye and skin and is caused by deficiency of fatty aldehyde dehydrogenase. Our recent finding of a profoundly disturbed brain tissue lipidome in SLS prompted us to search for similar biomarkers in plasma as no functional test in blood is available for SLS.

Methods and results

We performed plasma lipidomics and used a newly developed bioinformatics tool to mine the untargeted part of the SLS plasma and brain lipidome to search for SLS biomarkers. Plasma lipidomics showed disturbed ether lipid metabolism in known lipid classes. Untargeted lipidomics of both plasma and brain (white and grey matter) uncovered two new endogenous lipid classes highly elevated in SLS. The first biomarker group were alkylphosphocholines/ethanolamines containing different lengths of alkyl-chains where some alkylphosphocholines were > 600-fold elevated in SLS plasma. The second group of biomarkers were a set of 5 features of unknown structure. Fragmentation studies suggested that they contain ubiquinol and phosphocholine and one feature was also found as a glucuronide conjugate in plasma. The plasma features were highly distinctive for SLS with levels >100–1000-fold the level in controls, if present at all. We speculate on the origin of the alkylphosphocholines/ethanolamines and the nature of the ubiquinol-containing metabolites.

Conclusions

The metabolites identified in this study represent novel endogenous lipid classes thus far unknown in humans. They represent the first plasma metabolite SLS-biomarkers and may also yield more insight into SLS pathophysiology.

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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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