嗜酸性粒细胞食管炎中调节性 T 细胞增加并与肥大细胞相关

IF 3.3 3区医学 Q2 CLINICAL NEUROLOGY

Journal of Neurogastroenterology and Motility Pub Date : 2024-01-30 DOI:10.5056/jnm23040

Junji Chen, Tadayuki Oshima, Toshihiko Tomita, Hirokazu Fukui, Shinichiro Shinzaki

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引用次数: 0

摘要

背景/目的：近年来，嗜酸性粒细胞食管炎（EoE）的发病率不断上升。调节性 T 细胞（Tregs）在食管炎中的作用及其与其他炎症细胞的相关性仍不清楚。我们旨在阐明调节性 T 细胞在食管炎患者中的作用及其与炎症细胞的相关性：方法：对对照组和咽喉炎患者治疗前后的活检组织进行分析。通过苏木精和伊红染色评估嗜酸性粒细胞浸润情况。免疫组化染色检查 T 细胞、Tregs 和肥大细胞的浸润情况。通过逆转录定量聚合酶链反应评估趋化因子的基因表达：结果：EoE 患者食管上皮层中的 Tregs 和肥大细胞增多。治疗后，当组织学缓解时，Tregs 和肥大细胞减少。Tregs的浸润与嗜酸性粒细胞和肥大细胞的数量显著相关。嗜酸性粒细胞增多症患者的 Filaggrin mRNA 在治疗前减少，而在治疗后上调，即使未达到组织学缓解也是如此：结论：EoE 患者食管上皮细胞中的 Tregs 增加，并与肥大细胞浸润相关。

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Regulatory T cells Are Increased and Correlate With Mast Cells in Eosinophilic Esophagitis.

Background/aims: The incidence of eosinophilic esophagitis (EoE) has been increasing recently. The role of regulatory T cells (Tregs) and correlations with other inflammatory cells in EoE remain unknown. We aim to clarify the role of Tregs and their correlations with inflammatory cells in EoE patients.

Methods: Biopsies from controls and EoE patients before and after treatments were analyzed. Eosinophil infiltration was evaluated by hematoxylin and eosin staining. Immunohistochemical staining was performed to examine infiltration of T cells, Tregs, and mast cells. Gene expressions of chemokines were evaluated by reverse transcription-quantitative polymerase chain reaction.

Results: Tregs and mast cells were increased in the esophageal epithelial layers of EoE patients. After treatments, Tregs and mast cells were decreased when histologic remission was achieved. Infiltration of Tregs correlated significantly with numbers of eosinophils and mast cells. Filaggrin mRNA was decreased in patients with EoE before treatment and upregulated after treatment, even when histologic remission was not achieved.

Conclusions: Tregs were increased in esophageal epithelium of patients with EoE, and correlated with mast cell infiltration.

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来源期刊

Journal of Neurogastroenterology and Motility GASTROENTEROLOGY & HEPATOLOGY-CLINICAL NEUROLOGY

CiteScore

6.30

自引率

8.80%

发文量

期刊介绍： Journal of Neurogastroenterology and Motility (J Neurogastroenterol Motil) is a joint official journal of the Korean Society of Neurogastroenterology and Motility, the Thai Neurogastroenterology and Motility Society, the Japanese Society of Neurogastroenterology and Motility, the Indian Motility and Functional Disease Association, the Chinese Society of Gastrointestinal Motility, the South East Asia Gastro-Neuro Motility Association, the Taiwan Neurogastroenterology and Motility Society and the Asian Neurogastroenterology and Motility Association, launched in January 2010 after the title change from the Korean Journal of Neurogastroenterology and Motility, published from 1994 to 2009.