Bhawna Sharma, Pankaj Gupta, Largee Biswas, Anita Kamra Verma, Arif Mohammad Pasha, Prasad Thota, Bikash Medhi
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Further, ROT 1 mg/kg s.c. and EA 3 and 10 mg/kg p.o. were given to rats on a daily basis for 21 days, and the following parameters were assessed: (i) neurobehavioral studies, (ii) oxidative stress markers, (iii) neuroinflammatory markers, (iv) neurotransmitters, and (v) histopathological study.</p><p><strong>Results: </strong>The cell viability assay revealed that EA showed protection against ROT-induced toxicity in N2a cells, which was confirmed by a cell morphology study. EA decreased oxidative stress and % DNA fragmentation significantly. EA also prevented ROT-induced motor impairment and altered levels of oxidative stress markers, neurotransmitters, and neuroinflammatory markers significantly. When compared to the ROT group, a histological investigation of the EA group showed partial neuronal loss with the existence of intact neurons in between the vacuolated gaps.</p><p><strong>Conclusion: </strong>This study revealed that EA possesses profound neuroprotective properties in in vitro and in vivo studies. 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引用次数: 0
摘要
研究目的本研究旨在探讨芥酸(EA)作为一种ω-9单不饱和脂肪酸作为神经保护剂的潜力:本研究针对 N2a 细胞系和鱼藤酮(ROT)诱导的帕金森病模型对 EA 的神经保护潜力进行了研究。N2a 细胞系用胎牛血清、青霉素和链霉素辅以 Dulbecco's Modified Eagle's Medium 培养,并进行了以下检测:(i) MTT、(ii) 生物相容性、(iii) DCFDA 和 (iv) 二苯胺。还进行了细胞形态学研究。此外,每天给大鼠注射 ROT 1 mg/kg s.c.和 EA 3 和 10 mg/kg p.o.,连续 21 天,并评估以下参数:(i) 神经行为研究;(ii) 氧化应激标记;(iii) 神经炎症标记;(iv) 神经递质;(v) 组织病理学研究:细胞活力测定显示,EA 对 ROT 诱导的 N2a 细胞毒性具有保护作用,细胞形态学研究也证实了这一点。EA 能显著降低氧化应激和 DNA 断裂率。EA 还能防止 ROT 引起的运动损伤,并明显改变氧化应激标记物、神经递质和神经炎症标记物的水平。与 ROT 组相比,EA 组的组织学调查显示部分神经元缺失,空泡间隙中存在完整的神经元:本研究揭示了 EA 在体外和体内研究中具有深远的神经保护特性。我们还可以开展更多的研究,探讨 EA 的神经保护机制。
Neuroprotective potential of erucic acid via inhibition of N2a cell lines and rotenone induced Parkinson's disease rat model.
Objective: The objective of this study was to investigate the potential for erucic acid (EA), an omega-9 monounsaturated fatty acid, to act as a neuroprotective agent.
Materials and methods: In this study, EA was investigated against N2a cell lines and a rotenone (ROT)-induced model of Parkinson's disease for its neuroprotective potential. The N2a cell line was incubated with fetal bovine serum, penicillin, and streptomycin supplemented with Dulbecco's Modified Eagle's Medium, and the following assays were carried out: (i) MTT, (ii) biocompatibility, (iii) DCFDA, and (iv) diphenylamine. A cell morphology study was also performed. Further, ROT 1 mg/kg s.c. and EA 3 and 10 mg/kg p.o. were given to rats on a daily basis for 21 days, and the following parameters were assessed: (i) neurobehavioral studies, (ii) oxidative stress markers, (iii) neuroinflammatory markers, (iv) neurotransmitters, and (v) histopathological study.
Results: The cell viability assay revealed that EA showed protection against ROT-induced toxicity in N2a cells, which was confirmed by a cell morphology study. EA decreased oxidative stress and % DNA fragmentation significantly. EA also prevented ROT-induced motor impairment and altered levels of oxidative stress markers, neurotransmitters, and neuroinflammatory markers significantly. When compared to the ROT group, a histological investigation of the EA group showed partial neuronal loss with the existence of intact neurons in between the vacuolated gaps.
Conclusion: This study revealed that EA possesses profound neuroprotective properties in in vitro and in vivo studies. Additional research can be carried out to study the mechanism of EA with respect to its neuroprotective potential.
期刊介绍:
Indian Journal of Pharmacology accepts, in English, review articles, articles for educational forum, original research articles (full length and short communications), letter to editor, case reports and interesting fillers. Articles concerning all aspects of pharmacology will be considered. Articles of general interest (e.g. methods, therapeutics, medical education, interesting websites, new drug information and commentary on a recent topic) are also welcome.