出生后内脏胸膜拓扑秩序的演变

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Betty S. Liu, Ali B. Ali, Stacey P. Kwan, Jennifer M. Pan, Willi L. Wagner, Hassan A. Khalil, Zi Chen, Maximilian Ackermann, Steven J. Mentzer
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引用次数: 0

摘要

背景:上皮细胞形状的变化反映了细胞的最佳排列和表面自由能的最小化,同时也反映了细胞与细胞之间的相互作用、细胞增殖和细胞骨架的重新排列:在此,我们研究了大鼠出生后 15 天内的胸膜结构。胸膜分离和图像分割后,分析表明从出生后第 1 天(P1)到第 15 天,上皮细胞的排列顺序不断变化。在出生后第1天,观察到表面积最大、形状变化最大的细胞。相反,表面积最小、形状最一致的细胞出现在 P15。多边形细胞几何形状的比较显示,随着六边形(六面)以及五面和七面多边形数量的增加,细胞几何形状逐渐优化。利用沃罗诺网格和小图形图案频率对上皮组织进行的分析表明,其发育路径与数学和自然参考路径截然不同。细胞连通性的图论分析表明,从P1到P15,网络异质性和聚类系数逐渐降低:我们得出结论:从 P1 到 P15,大鼠胸膜结构发生了显著变化。结论:我们得出结论,大鼠胸膜结构从 P1 到 P15 会发生显著变化。此外,基于几何和网络的方法可提供这些发育变化的定量特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evolving topological order in the postnatal visceral pleura

Background

Changes in epithelial cell shape reflects optimal cell packing and the minimization of surface free energy, but also cell–cell interactions, cell proliferation, and cytoskeletal rearrangements.

Results

Here, we studied the structure of the rat pleura in the first 15 days after birth. After pleural isolation and image segmentation, the analysis demonstrated a progression of epithelial order from postnatal day 1 (P1) to P15. The cells with the largest surface area and greatest shape variability were observed at P1. In contrast, the cells with the smallest surface area and most shape consistency were observed at P15. A comparison of polygonal cell geometries demonstrated progressive optimization with an increase in the number of hexagons (six-sided) as well as five-sided and seven-sided polygons. Analysis of the epithelial organization with Voronoi tessellations and graphlet motif frequencies demonstrated a developmental path strikingly distinct from mathematical and natural reference paths. Graph Theory analysis of cell connectivity demonstrated a progressive decrease in network heterogeneity and clustering coefficient from P1 to P15.

Conclusions

We conclude that the rat pleura undergoes a striking change in pleural structure from P1 to P15. Further, a geometric and network-based approach can provide a quantitative characterization of these developmental changes.

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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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