远程感染患者的登革热基因组持续存在

IF 0.4 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Asian Biomedicine Pub Date : 2023-12-28 DOI:10.2478/abm-2023-0072

Soraya Thaivanich, Jirayu Visuthranukul, Kesinee Arunyingmongkol, Udomsak Bunworasate, Padet Siriyasatien, Wanla Kulwichit

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引用次数: 0

摘要

背景登革病毒感染是一种令人费解的疾病。传统上认为它是一种自限性和非持续性疾病。目的我们报告了一例在远程感染者的造血细胞中检测到持续性登革病毒基因组的病例。方法一名多发性骨髓瘤缓解期患者准备进行外周血干细胞（PBSC）移植。收集血浆和 G-CSF 刺激动员的 PBSCs。对刺激前和刺激后的血液样本进行登革热特异性逆转录聚合酶链反应（RT-PCR）。在干细胞动员前后，通过酶联免疫吸附试验和中和试验测定了抗登革热抗体。结果仅在 G-CSF 刺激后标本的 PBSC 中检测到病毒基因组。在干细胞动员前后，通过 ELISA 和中和检测，抗登革热抗体分别为阴性和阳性。结论我们的发现揭示了一种持续性感染。该菌株是否以及如何与后续血清型相互作用仍有待阐明。

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Persistence of dengue genome in a remotely infected patient

Background Dengue virus infection is an intriguing illness. It is traditionally thought of as a self-limited and nonpersistent disease. Objectives We report a case with persistent dengue virus genome detectable in hematopoietic cells of a person with remote infection. Methods A patient with multiple myeloma in remission was prepared for peripheral blood stem cell (PBSC) transplantation. Plasma and G-CSF-stimulated, mobilized PBSCs were collected. Dengue-specific reverse transcription polymerase chain reaction (RT-PCR) was performed in both pre- and post-stimulated blood specimens. Anti-dengue antibodies by ELISA and by neutralization assay were measured before and after the stem cell mobilization. Results The viral genome was detected only in the PBSC of the post-G-CSF-stimulated specimens. Anti-dengue antibodies were negative and positive, by ELISA and neutralization assays, respectively, both before and after stem cell mobilization. Conclusion Our findings reveal a persistent infection. Whether and how this strain may interact with subsequent serotype(s) remains to be elucidated.

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来源期刊

Asian Biomedicine 医学-医学：研究与实验

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Asian Biomedicine: Research, Reviews and News (ISSN 1905-7415 print; 1875-855X online) is published in one volume (of 6 bimonthly issues) a year since 2007. [...]Asian Biomedicine is an international, general medical and biomedical journal that aims to publish original peer-reviewed contributions dealing with various topics in the biomedical and health sciences from basic experimental to clinical aspects. The work and authorship must be strongly affiliated with a country in Asia, or with specific importance and relevance to the Asian region. The Journal will publish reviews, original experimental studies, observational studies, technical and clinical (case) reports, practice guidelines, historical perspectives of Asian biomedicine, clinicopathological conferences, and commentaries Asian biomedicine is intended for a broad and international audience, primarily those in the health professions including researchers, physician practitioners, basic medical scientists, dentists, educators, administrators, those in the assistive professions, such as nurses, and the many types of allied health professionals in research and health care delivery systems including those in training.