进食时 Rhipicephalus microplus 中肠的纵向转录组分析

IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES
Stephen Lu , Jéssica Waldman , Luís Fernando Parizi , Itabajara da Silva Vaz Junior , Lucas Tirloni
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引用次数: 0

摘要

Rhipicephalus microplus 是一种对宿主具有高度特异性的蜱,主要以牛为食,对畜牧业生产构成严重威胁。对蜱虫生理机能的研究对于确定潜在的蜱虫控制目标至关重要。尤其令人感兴趣的是,成年雌性蜱在进食过程中中肠会显著扩张,导致体重增加 100 多倍。除了储存和消化血食的功能外,蜱的中肠在获取和传播病原体方面也起着至关重要的作用。然而,我们对蜱中肠生理学的了解仍然有限。在这项研究中,我们对在不同摄食阶段收集到的成年雌性 R. microplus 蜱的中肠进行了全面的纵向转录组分析,提供了该器官随着摄食进展的转录调控概况。通过采用从头组装方法和编码序列(CDS)提取方法,共鉴定出 60,599 个潜在的 CDS。在准备功能注释和差异表达分析时,提取了在至少一种生物条件下平均每百万转录本(TPM)≥3的转录本。通过这一筛选过程,共获得了 10,994 个 CDS,并将其分为 24 个功能类别。值得注意的是,我们的差异表达分析揭示了三种主要的转录特征。第一种是慢食阶段,最丰富的功能类是 "蛋白质合成 "和 "分泌 "类,反映了蜱中肠的高度活跃状态。第二个图谱部分反映了快速进食阶段,在该阶段观察到大量差异表达的转录本。最后,第三个转录特征代表了脱落后的蜱虫。值得注意的是,在脱落后 48 小时(hpd)内观察到的调节转录本数量最多,但在 168 小时(hpd)内没有观察到重大差异。总之,本文提供的数据从时间角度揭示了蜱的中肠生理学,有助于确定开发抗蜱控制策略的潜在目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A longitudinal transcriptomic analysis of Rhipicephalus microplus midgut upon feeding

Rhipicephalus microplus, a highly host-specific tick that primarily feeds on cattle, posing a significant threat to livestock production. The investigation of tick physiology is crucial for identifying potential targets in tick control. Of particular interest adult female ticks undergo a significant expansion of the midgut during feeding, leading to an over 100-fold increase in body weight. Beyond the functions of storing and digesting blood meals, the tick midgut plays a crucial role in acquiring and transmitting pathogens. However, our understanding of tick midgut physiology remains limited. In this study we conducted a comprehensive longitudinal transcriptome analysis of the midgut from adult female R. microplus ticks collected at various feeding stages, providing an overview of the transcriptional modulation in this organ as feeding progress. By employing a de novo assembly approach followed by coding-sequences (CDS) extraction, 60,599 potential CDS were identified. In preparation for functional annotation and differential expression analysis, transcripts that showed an average transcript per million (TPM) ≥ 3 in at least one of the biological conditions were extracted. This selection process resulted in a total of 10,994 CDS, which were categorized into 24 functional classes. Notably, our differential expression analysis revealed three main transcriptional profiles. In the first one, representing the slow-feeding stage, the most abundant functional classes were the “protein synthesis” and “secreted” groups, reflecting the highly active state of the tick midgut. The second profile partially accounts for the rapid-feeding stage, in which a high number of differentially expressed transcripts was observed. Lastly, the third transcriptional profile represents post-detached ticks. Notably the highest number of modulated transcripts was observed up to 48 h post-detachment (hpd), however no major differences was observed up to 168 hpd. Overall, the data presented here offers a temporal insight into tick midgut physiology, contributing to the identification of potential targets for the development of anti-tick control strategies.

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来源期刊
Ticks and Tick-borne Diseases
Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY
CiteScore
6.90
自引率
12.50%
发文量
185
审稿时长
6-12 weeks
期刊介绍: Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.
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