{"title":"诱导 ICR 小鼠不良妊娠结局的妊娠相关疟疾模型","authors":"Yingying Zhang , Zhiming Liang , Haoyu Xing , Chuyi Yu , Jianming Liang , Qin Xu , Jianping Song , Zhouqing He","doi":"10.1016/j.exppara.2023.108686","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Based on understanding of placental pathological features and safe medication in pregnancy-associated malaria (PAM), establishment of a stable pregnant mouse infection model with <em>Plasmodium</em> was urgently needed.</p></div><div><h3>Methods</h3><p><span>ICR mice with vaginal plugs detected were randomly divided into post-pregnancy infection (Malaria</span><sup>+</sup>) and uninfected pregnancy (Malaria<sup>−</sup>) cohorts. Age-matched mice that had not been mated were infected as pre-pregnancy infection group (Virgin control), which were subsequently mated with ICR males. All mice were inoculated with 1 × 10<sup>6</sup> <span><em>Plasmodium berghei</em></span> ANKA-infected RBCs by intraperitoneal injection, and the same amount of saline was given to Malaria<sup>−</sup> group. We recorded the incidence of adverse pregnancy outcomes and the amounts of offspring in each group.</p></div><div><h3>Results</h3><p>The Virgin group mice were unable to conceive normally, and vaginal bleeding, abortion, or stillbirth appeared in the Malaria<sup>+</sup> group. The incidence of adverse pregnancy outcomes was extremely high and statistically significant compared with the control (Malaria<sup>−</sup>) group (<em>P</em> < 0.05), of which placenta exhibited pathological features associated with human gestational malaria.</p></div><div><h3>Conclusions</h3><p>The intraperitoneal injection of 1 × 10<sup>6</sup> <em>Plasmodium berghei</em> ANKA-infected RBCs could establish a model of pregnancy-associated malaria in ICR mouse.</p></div>","PeriodicalId":12117,"journal":{"name":"Experimental parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A model of pregnancy-associated malaria for inducing adverse pregnancy outcomes in ICR mouse\",\"authors\":\"Yingying Zhang , Zhiming Liang , Haoyu Xing , Chuyi Yu , Jianming Liang , Qin Xu , Jianping Song , Zhouqing He\",\"doi\":\"10.1016/j.exppara.2023.108686\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Based on understanding of placental pathological features and safe medication in pregnancy-associated malaria (PAM), establishment of a stable pregnant mouse infection model with <em>Plasmodium</em> was urgently needed.</p></div><div><h3>Methods</h3><p><span>ICR mice with vaginal plugs detected were randomly divided into post-pregnancy infection (Malaria</span><sup>+</sup>) and uninfected pregnancy (Malaria<sup>−</sup>) cohorts. Age-matched mice that had not been mated were infected as pre-pregnancy infection group (Virgin control), which were subsequently mated with ICR males. All mice were inoculated with 1 × 10<sup>6</sup> <span><em>Plasmodium berghei</em></span> ANKA-infected RBCs by intraperitoneal injection, and the same amount of saline was given to Malaria<sup>−</sup> group. We recorded the incidence of adverse pregnancy outcomes and the amounts of offspring in each group.</p></div><div><h3>Results</h3><p>The Virgin group mice were unable to conceive normally, and vaginal bleeding, abortion, or stillbirth appeared in the Malaria<sup>+</sup> group. The incidence of adverse pregnancy outcomes was extremely high and statistically significant compared with the control (Malaria<sup>−</sup>) group (<em>P</em> < 0.05), of which placenta exhibited pathological features associated with human gestational malaria.</p></div><div><h3>Conclusions</h3><p>The intraperitoneal injection of 1 × 10<sup>6</sup> <em>Plasmodium berghei</em> ANKA-infected RBCs could establish a model of pregnancy-associated malaria in ICR mouse.</p></div>\",\"PeriodicalId\":12117,\"journal\":{\"name\":\"Experimental parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-12-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014489423002278\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014489423002278","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
A model of pregnancy-associated malaria for inducing adverse pregnancy outcomes in ICR mouse
Background
Based on understanding of placental pathological features and safe medication in pregnancy-associated malaria (PAM), establishment of a stable pregnant mouse infection model with Plasmodium was urgently needed.
Methods
ICR mice with vaginal plugs detected were randomly divided into post-pregnancy infection (Malaria+) and uninfected pregnancy (Malaria−) cohorts. Age-matched mice that had not been mated were infected as pre-pregnancy infection group (Virgin control), which were subsequently mated with ICR males. All mice were inoculated with 1 × 106Plasmodium berghei ANKA-infected RBCs by intraperitoneal injection, and the same amount of saline was given to Malaria− group. We recorded the incidence of adverse pregnancy outcomes and the amounts of offspring in each group.
Results
The Virgin group mice were unable to conceive normally, and vaginal bleeding, abortion, or stillbirth appeared in the Malaria+ group. The incidence of adverse pregnancy outcomes was extremely high and statistically significant compared with the control (Malaria−) group (P < 0.05), of which placenta exhibited pathological features associated with human gestational malaria.
Conclusions
The intraperitoneal injection of 1 × 106Plasmodium berghei ANKA-infected RBCs could establish a model of pregnancy-associated malaria in ICR mouse.
期刊介绍：
Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.
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