从人类肾酶的氨基酸序列中寻找潜在的降血压肽,并在该蛋白质的蛋白水解片段中对其进行鉴定。

Q3 Biochemistry, Genetics and Molecular Biology
V I Fedchenko, A V Veselovsky, A T Kopylov, A E Medvedev
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引用次数: 0

摘要

肾酶(RNLS)是 2005 年发现的一种分泌蛋白。它在调节血压方面发挥着重要作用。两个独立实验室的研究表明,服用纯化的重组 RNLS 可降低实验动物的血压。然而,RNLS 的降压作用机制仍不清楚,尤其是在该蛋白的催化模式发生转变的背景下。此外,越来越多的证据表明,用酶免疫测定法检测到的内源性血浆/血清 RNLS 并不是分泌到细胞外空间的完整蛋白质,外源性重组 RNLS 在与人体血浆样本短期孵育过程中会被有效裂解。这表明,RNLS 的降压作用可能是由蛋白水解过程中形成的肽引起的。根据对 RNLS 潜在裂解位点的生物信息学分析结果(Fedchenko 等人,《医学假设》,2022 年;DOI: 10.1016/j.mehy.2022.110895),在 RNLS 序列中发现了一些短肽,它们与已知的血管紧张素转换酶多肽抑制剂片段相似。其中一些短肽是较大的 RNLS 肽的一部分,在 RNLS 被糜蛋白酶和胰蛋白酶(其次是胰酶)裂解时形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The search for potential hypotensive peptides in the amino acid sequence of human renalase and their identification in proteolytic fragments of this protein.

Renalase (RNLS) is a secretory protein discovered in 2005. It plays an important role in the regulation of blood pressure. Studies by two independent laboratories have shown that administration of purified recombinant RNLS reduced blood pressure in experimental animals. However, the mechanisms of the antihypertensive effect of RNLS still remain unclear, especially in the context of the shift in the catalytic paradigm of this protein. In addition, there is growing evidence that endogenous plasma/serum RNLS, detected by enzyme immunoassay, is not an intact protein secreted into the extracellular space, and exogenous recombinant RNLS is effectively cleaved during short-term incubation with human plasma samples. This suggests that the antihypertensive effect of RNLS may be due to peptides formed during proteolytic processing. Based on the results of a bioinformatics analysis of potential RNLS cleavage sites (Fedchenko et al., Medical Hypotheses, 2022; DOI: 10.1016/j.mehy.2022.110895), a number of short peptides have been identified in the RNLS sequence that show similarity to fragments of known peptide inhibitors of angiotensin-converting enzyme. Some of them were found as a part of larger RNLS peptides, formed during RNLS cleavage by chymotrypsin and, and to a lesser extent, by trypsin.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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