大鼠去内皮化主动脉的血小板积累和周转。

P D Winocour, R L Kinlough-Rathbone, M Richardson, J F Mustard
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引用次数: 0

摘要

先前的研究表明,经球囊导管去内皮化的兔主动脉内皮下层迅速被单层血小板覆盖;60分钟后,少量额外的血小板积累,尽管大多数血小板在4天内从损伤表面消失,但在重新内皮化之前有很大的延迟。我们用球囊导管检查了去内皮大鼠主动脉的血小板积累动态,以确定对这种类型损伤的反应是否与兔主动脉相似。当在主动脉去内皮化之前注射51cr -血小板时,在前15分钟内,大鼠内皮下层积聚了25,500 +/- 2,750个血小板/mm2。60和92小时后,表面保留的血小板减少(分别为13,740 +/- 2,400和5,020 +/- 1,330个血小板/mm2)。大鼠损伤后30min注射51cr -血小板,30min内血小板累积量为8610 +/- 1230血小板/mm2。到第4天,大鼠主动脉在30分钟内没有明显积聚新注射的血小板,但在24小时内积累了20,600 +/- 3,490血小板/mm2。形态学上,损伤后4天,大鼠主动脉非内皮化区几乎完全被血小板覆盖。损伤后第14天,大鼠主动脉未积累新注入的血小板,形态学上,主动脉表面无血小板存在,几乎是再内皮化。因此,在大鼠中,与家兔一样,血小板在去内皮化的主动脉上迅速积累,并且在损伤后不久,吸引新引入血小板的能力大大降低。然而,与家兔相比,大鼠去内皮化主动脉在损伤后4天内仍对新血小板有吸引力,但比损伤时更弱。此外,与家兔相比,大鼠主动脉损伤后14天,表面几乎完全再内皮化。因此,血小板与去内皮化血管的相互作用存在物种差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet accumulation and turnover on de-endothelialized aortae in rats.

Previous studies indicate that the subendothelium of rabbit aortae de-endothelialized with a balloon catheter rapidly becomes covered with a monolayer of platelets; after 60 min few additional platelets accumulate and although most platelets are lost from the injured surface by 4 days, there is a substantial delay before re-endothelialization. We examined the dynamics of platelet accumulation on rat aortae de-endothelialized with a balloon catheter to determine if the response to this type of injury is similar to rabbit aortae. When 51Cr-platelets were injected prior to aortic de-endothelialization, 25,500 +/- 2,750 platelets/mm2 accumulated on rat subendothelium in the first 15 min. After 60 and 92 h, fewer platelets remained on the surface (13,740 +/- 2,400 and 5,020 +/- 1,330 platelets/mm2, respectively). When 51Cr-platelets were injected into rats 30 min after injury, platelet accumulation in a 30-min period was 8,610 +/- 1,230 platelets/mm2. By 4 days rat aortae did not accumulate newly injected platelets significantly in a 30-min period, but in a 24-h period 20,600 +/- 3,490 platelets/mm2 accumulated. Morphologically, the non-endothelialized areas of rat aortae were almost completely covered with platelets 4 days after injury. Fourteen days after injury, rat aortae did not accumulate newly injected platelets and, morphologically, no platelets were present on the surface which was almost re-endothelialized. Thus, in rats, as with rabbits, platelets rapidly accumulate on de-endothelialized aortae and the ability to attract newly introduced platelets is considerably reduced shortly after injury. In contrast to rabbits, however, de-endothelialized aortae in rats remain attractive to new platelets up to 4 days following injury, but less so than at the time of injury. Also, in contrast to rabbits, 14 days after injury to rat aortae the surface is almost completely re-endothelialized. Thus, there are species differences in platelet interactions with de-endothelialized vessels.

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