厄贝沙坦可减轻体外和体内脂多糖诱发的肺损伤

IF 1.4 4区 医学 Q4 PHYSIOLOGY
Zhongyuan Zhang, Wei Wang
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引用次数: 0

摘要

急性肺损伤(ALI)是一种破坏性肺部疾病,发病率和致死率都很高。厄贝沙坦(IRB)是一种血管紧张素 II 受体阻滞剂,有人认为它能防止油酸诱导的 ALI。为此,本研究的重点是确定 IRB 在 ALI 中的作用,并找出其可能的作用机制。首先,细胞计数试剂盒-8(CCK-8)评估了暴露于升高浓度 IRB 的人肺微血管内皮细胞(HPMVECs)的活力。HPMVEC损伤模型和脂多糖(LPS)诱导的小鼠ALI模型均由IRB预处理。在体外,用 CCK-8 检测法评估细胞活力,用 LDH 检测试剂盒检测乳酸脱氢酶(LDH)的释放。酶联免疫吸附试验(ELISA)和 Western 印迹法检测了炎症因子的表达水平。异硫氰酸荧光素-葡聚糖用于评估 HPMVEC 的通透性。Western 印迹检查了粘附蛋白和紧密连接蛋白的表达。在体内,苏木精和伊红染色评估肺组织损伤,并测量肺湿/干(W/D)重量。ELISA 分析了血清和支气管肺泡灌洗液(BALF)中炎症因子的水平,Western 印迹检查了炎症因子的表达。用细胞计数器测定 BALF 中的总细胞数、中性粒细胞数和巨噬细胞数。用伊文思蓝白蛋白检测肺毛细血管通透性,用双喹啉酸法测量 BALF 中的总蛋白浓度。免疫荧光检测和 Western 印迹检测了肺组织中粘附蛋白和紧密连接蛋白的表达。实验结果表明,IRB 可剂量依赖性地增强 LPS 挑战的 HPMVECs 的活力,同时降低 LDH 释放、炎症反应和通透性。此外,IRB 处理后,体内 LPS 刺激的肺组织损伤、肺水肿、炎症反应和肺毛细血管通透性均得到逆转。总之,IRB 治疗可对 LPS 引发的 ALI 起到保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Irbesartan eases lipopolysaccharide-induced lung injury In Vitro and In Vivo.

Acute lung injury (ALI) is classified as a devastating pulmonary disorder contributing to significant incidence and fatality rate. Irbesartan (IRB) is an angiotensin II receptor blocker that has been proposed to protect against oleic acid-induced ALI. To this end, the current study is concentrated on ascertaining the role of IRB in ALI and figuring out the probable action mechanism. First, cell counting kit-8 (CCK-8) appraised the viability of human pulmonary microvascular endothelial cells (HPMVECs) exposed to ascending concentrations of IRB. HPMVEC injury model and a mouse model of ALI induced by lipopolysaccharide (LPS) were pretreated by IRB. In vitro, cell viability was estimated by CCK-8 assay, and lactate dehydrogenase (LDH) release was tested by LDH assay kit. Enzyme-linked immunosorbent assay (ELISA) and Western blotting estimated the expression levels of inflammatory factors. Fluorescein isothiocyanate-dextran was used to assess HPMVEC permeability. Western blotting examined the expression of adherent and tight junction proteins. In vivo, hematoxylin and eosin staining evaluated lung tissue damage and lung wet/dry (W/D) weight was measured. ELISA analyzed the levels of inflammatory factors in the serum and bronchoalveolar lavage fluid (BALF), and Western blotting examined the expression of inflammatory factors. The total cell, neutrophil, and macrophage numbers in BALF were determined using a cell counter. Lung capillary permeability was assayed by Evans blue albumin and total protein concentration in BALF was measured using bicinchoninic acid method. Immunofluorescence assay and Western blotting examined the expression of adherent and tight junction proteins in lung tissues. It was observed that IRB dose-dependently enhanced the viability while reduced LDH release, inflammatory response as well as permeability in LPS-challenged HPMVECs in vitro. In addition, LPS-stimulated lung tissue damage, pulmonary edema, inflammatory response as well as lung capillary permeability in vivo were all reversed following IRB treatment. Collectively, IRB treatment might elicit protective behaviors against LPS-triggered ALI.

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来源期刊
CiteScore
2.30
自引率
5.60%
发文量
36
审稿时长
6-12 weeks
期刊介绍: Chinese Journal of Physiology is a multidisciplinary open access journal. Chinese Journal of Physiology (CJP) publishes high quality original research papers in physiology and pathophysiology by authors all over the world. CJP welcomes submitted research papers in all aspects of physiology science in the molecular, cellular, tissue and systemic levels. Multidisciplinary sciences with a focus to understand the role of physiology in health and disease are also encouraged. Chinese Journal of Physiology accepts fourfold article types: Original Article, Review Article (Mini-Review included), Short Communication, and Editorial. There is no cost for readers to access the full-text contents of publications.
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