用单色 Cerrena 的 CE15 葡萄糖醛酸酯酶进行酯交换反应揭示了底物偏好。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-02-01 Epub Date: 2023-12-27 DOI:10.1007/s10529-023-03456-x
Valentina Perna, Jane Wittrup Agger
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引用次数: 0

摘要

目的:葡萄糖醛酸酯酶(GE,CE15 家族)可催化木质素-碳水化合物复合物(LCC)中酯连接的裂解,本研究展示了与一种来自 Cerrena unicolor 的真菌 GE(CuGE)的酯化反应如何揭示该酶对酯键中醇部分的偏好:这种对醇的偏好与木质素分子上的酯键位置有关,并对酶与木质素的潜在相互作用方式产生影响。目前还不清楚酶究竟偏好α-苄基还是γ-苄基位置。通过向酶提供供体底物(葡萄糖醛酸甲酯或 4-O-甲基-葡萄糖醛酸甲酯)和两种受体分子(苄醇或 3-苯基-1-丙醇)中的一种,我们证明该酶可以进行酯交换反应,这也是评估酶对醇的偏好的一种方法:结论:CuGE 优先从 4-O-甲基葡萄糖醛酸甲酯和 3-苯基-1-丙醇形成 γ-酯,该酶对底物的偏好主要取决于葡萄糖醛酸供体上是否存在 4-O-甲基化,其次取决于醇的类型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transesterification with CE15 glucuronoyl esterase from Cerrena unicolor reveals substrate preferences.

Transesterification with CE15 glucuronoyl esterase from Cerrena unicolor reveals substrate preferences.

Purpose: Glucuronoyl esterases (GE, family CE15) catalyse the cleavage of ester linkages in lignin-carbohydrate complexes (LCCs), and this study demonstrate how transesterification reactions with a fungal GE from Cerrena unicolor (CuGE) can reveal the enzyme's preference for the alcohol-part of the ester-bond.

Methods: This alcohol-preference relates to where the ester-LCCs are located on the lignin molecule, and has consequences for how the enzymes potentially interact with lignin. It is unknown exactly what the enzymes prefer; either the α-benzyl or the γ-benzyl position. By providing the enzyme with a donor substrate (the methyl ester of either glucuronate or 4-O-methyl-glucuronate) and either one of two acceptor molecules (benzyl alcohol or 3-phenyl-1-propanol) we demonstrate that the enzyme can perform transesterification and it serves as a method for assessing the enzyme's alcohol preferences.

Conclusion: CuGE preferentially forms the γ-ester from the methyl ester of 4-O-methyl-glucuronate and 3-phenyl-1-propanol and the enzyme's substrate preferences are primarily dictated by the presence of the 4-O-methylation on the glucuronoyl donor, and secondly on the type of alcohol.

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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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