{"title":"三维打印盒与纸质灵敏度传感器集成，用于构建检测登革热病毒的下一代传感工具，实现 plaspertronix 商品化","authors":"MohdRahil Hasan, Pradakshina Sharma, Saumitra Singh, Annu Mishra, Zaira Azmi, Jagriti Narang","doi":"10.1016/j.biosx.2023.100431","DOIUrl":null,"url":null,"abstract":"<p>The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization.</p>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 1","pages":""},"PeriodicalIF":10.6100,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D-printed cassette integrated with paper-based aptasensor for the construction of next-generation sensing tool to detect dengue virus towards plaspertronix-commercialization\",\"authors\":\"MohdRahil Hasan, Pradakshina Sharma, Saumitra Singh, Annu Mishra, Zaira Azmi, Jagriti Narang\",\"doi\":\"10.1016/j.biosx.2023.100431\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization.</p>\",\"PeriodicalId\":260,\"journal\":{\"name\":\"Biosensors and Bioelectronics: X\",\"volume\":\"25 1\",\"pages\":\"\"},\"PeriodicalIF\":10.6100,\"publicationDate\":\"2023-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics: X\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.biosx.2023.100431\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics: X","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.biosx.2023.100431","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
3D-printed cassette integrated with paper-based aptasensor for the construction of next-generation sensing tool to detect dengue virus towards plaspertronix-commercialization
The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization.
期刊介绍：
Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.
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