加速酶和通路原型开发的无细胞系统和基因生物传感器

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Wonhee Kim , Sohun Lee , Bong Hyun Sung , Jeong-Geol Na , Jeong Wook Lee
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引用次数: 0

摘要

无细胞系统与基因生物传感器的整合正在成为小分子检测的有利平台。这种生物传感器耦合无细胞系统结合了无细胞系统快速高效表达蛋白质的优势和基因生物传感器提供的原位检测能力,简化了化验和检测程序。此外,由于无细胞系统的开放环境提高了整体操作的简便性,因此该系统易于同时检测多种条件。在本综述中,我们将重点讨论利用无细胞生物传感器加速酶和通路原型开发,以及提高生物传感器灵敏度和特异性的策略。此外还介绍了高通量筛选工具,这些工具可以通过生成大量数据集来快速评估,从而扩展原型开发过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell-free systems and genetic biosensors for accelerating enzyme and pathway prototyping

Integration of cell-free systems with genetic biosensors is emerging as an advantageous platform for small molecule detection. This biosensor-coupled cell-free system simplifies an assay-and-detection procedure by combining the advantages of rapid and efficient protein expression through a cell-free system and the in situ detection capabilities provided by genetic biosensors. Moreover, this system is easy to assay multiple conditions at once, as the open environment of the cell-free systems enhances overall ease of handling. In this review, we focus on the acceleration of enzyme and pathway prototyping using cell-free biosensors, as well as strategies to improve the sensitivity and specificity of biosensors. High-throughput screening tools that can expand the prototyping process by generating massive data sets for rapid evaluation were also described.

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来源期刊
Current Opinion in Systems Biology
Current Opinion in Systems Biology Mathematics-Applied Mathematics
CiteScore
7.10
自引率
2.70%
发文量
20
期刊介绍: Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution
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