卡拉恰伊-切尔克斯共和国不同地区私人农庄小牛的隐孢子虫病

R. Akbaev, N. Babichev, Alena Zolotuhina
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引用次数: 0

摘要

隐孢子虫病是一种广泛存在的寄生虫(原生动物)疾病。研究目的研究卡拉恰伊-切尔克斯共和国境内部分地区私人农场条件下小牛隐孢子虫病的传播情况。材料和方法采用本地涂片法对 55 头 3 至 50 天的小牛(临床健康和患病,有腹泻症状)的粪便样本进行了检查,以确定是否存在隐孢子虫病的病原体。完成的涂片按照尼基福罗夫法固定,按照齐氏-奈尔森法用石碳酸品红染色,并在配有浸透镜的双目光学显微镜 "Micromed-3 "下进行检查。经 Ziehl-Neelsen 染色后,隐孢子虫卵囊呈现丰富的粉红色。最终诊断是根据检测到的这种入侵特征的卵囊确定的。使用 Krylov M.V. 的 "寄生原生动物决定因素 "和 Vershinin I.I. 的图谱对隐孢子虫进行鉴定。对 55 头犊牛的粪便样本涂片进行显微镜检查后发现,29 份涂片中含有隐孢子虫卵囊,因此,隐孢子虫的入侵程度(EI)为 52.7%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryptosporidiosis of calves in a private farmstead on the territory of different districts of the Karachay-Cherkess Republic
Cryptosporidiosis is a widespread parasitic (protozoal) disease. to which more than 170 species of animals are susceptible. Purpose of the study. To study the spread of cryptosporidiosis of calves in the conditions of private farmsteads in some areas on the territory of the Karachay-Cherkess Republic. Materials and methods. Fecal samples from 55 calves (clinically healthy and sick, with signs of diarrhea) aged 3 to 50 days were examined for the presence of the causative agent of cryptosporidiosis using the native smear method. The finished smears were fixed according to the Nikiforov method, stained with carbolic fuchsin according to Ziehl–Neelsen and examined under a binocular light microscope «Micromed-3» with an immersion lens. Cryptosporidium oocysts acquire a rich pink-red color when stained by Ziehl–Neelsen. The final diagnosis was established based on the detection of oocysts characteristic of this invasion. Cryptosporidium was identified using the «Determinant of parasitic protozoa» of Krylov M.V. and the atlas of Vershinin I.I. Results of the study. Microscopy of smears of faecal samples from 55 calves revealed cryptosporidium oocysts in 29 smears, thus, the extent of invasion (EI) was 52.7 %.
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