产前接触双酚 A 和/或邻苯二甲酸二乙基己酯会以性别和剂量依赖的方式改变大鼠后代的应激反应

IF 3.6 Q2 TOXICOLOGY
Amrita Kaimal, Jessica M. Hooversmith, A. Cherry, Jillian T. Garrity, Maryam H. Al Mansi, N. Martin, Hannah E. Buechter, Philip V. Holmes, P. MohanKumar, S. MohanKumar
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引用次数: 0

摘要

背景:产前暴露于干扰内分泌的化学物质(EDCs)与不良行为结果有关,但这些化学物质组合的影响尚不清楚。本研究旨在确定产前暴露于 EDCs 对雄性和雌性行为的剂量依赖性影响:方法:对怀孕的 Sprague-Dawley 大鼠分别口服载体、双酚 A(BPA)(5 μg/kg 体重/天)、低剂量(LD)邻苯二甲酸二乙基己酯(DEHP)(5 μg/kg 体重/天)、高剂量(HD)DEHP(7.在妊娠期的第 6-21 天,对后代进行双酚 A 和邻苯二甲酸二乙基己酯(DEHP)(5 微克/千克体重/天)、高剂量(HD)DEHP(7.成年后代在成年后接受开阔地试验(OFT)、高架加迷宫试验(EPM)和冲击探针防御掩埋试验(SPDB)。牺牲时收集身体、肾上腺和垂体的重量。测量血清中的皮质酮(CORT):结果:暴露于 EDC 的雌性后代在 OFT 中表现出抗焦虑效应,而雄性后代则不受影响。DEHP(HD)雄性后代在EPM中表现出雌性化行为。大多数暴露于 EDC 的雄性后代在 SPDB 中的埋葬程度较低,而雌性后代则表现出较低的冲击反应性,这表明在防御行为方面存在性别特异性适应不良改变。此外,在该试验中,DEHP(LD)雄性和雌性以及B + D(LD)雌性表现出更强的不稳定性。单独使用 DEHP (LD) 或与双酚 A 结合使用 DEHP (LD) 会导致肾上腺重量降低,但只有雄性后代会出现这种情况。最后,用 B + D 混合物(HD)处理的雌性后代体内 CORT 水平升高:结论:产前暴露于双酚 A、DEHP 或两者的混合物会以性别和剂量依赖的方式影响行为、CORT 水平和肾上腺重量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prenatal exposure to bisphenol A and/or diethylhexyl phthalate alters stress responses in rat offspring in a sex- and dose-dependent manner
Background: Prenatal exposures to endocrine disrupting chemicals (EDCs) are correlated with adverse behavioral outcomes, but the effects of combinations of these chemicals are unclear. The aim of this study was to determine the dose-dependent effects of prenatal exposure to EDCs on male and female behavior.Methods: Pregnant Sprague-Dawley rats were orally dosed with vehicle, bisphenol A (BPA) (5 μg/kg body weight (BW)/day), low-dose (LD) diethylhexyl phthalate (DEHP) (5 μg/kg BW/day), high-dose (HD) DEHP (7.5 mg/kg BW/day), a combination of BPA and LD-DEHP (B + D (LD)), or a combination of BPA and HD-DEHP (B + D (HD)) on gestational days 6–21. Adult offspring were subjected to the Open Field Test (OFT), Elevated Plus Maze (EPM), and Shock Probe Defensive Burying test (SPDB) in adulthood. Body, adrenal gland, and pituitary gland weights were collected at sacrifice. Corticosterone (CORT) was measured in the serum.Results: Female EDC-exposed offspring showed anxiolytic effects in the OFT, while male offspring were unaffected. DEHP (HD) male offspring demonstrated a feminization of behavior in the EPM. Most EDC-exposed male offspring buried less in the SPDB, while their female counterparts showed reduced shock reactivity, indicating sex-specific maladaptive alterations in defensive behaviors. Additionally, DEHP (LD) males and females and B + D (LD) females displayed increased immobility in this test. DEHP (LD) alone and in combination with BPA led to lower adrenal gland weights, but only in male offspring. Finally, females treated with a mixture of B + D (HD) had elevated CORT levels.Conclusion: Prenatal exposure to BPA, DEHP, or a mixture of the two, affects behavior, CORT levels, and adrenal gland weights in a sex- and dose-dependent manner.
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CiteScore
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