修剪处理对杜仲（E. ulmoides Oliver）生长特性和代谢物的影响

IF 2.4 2区农林科学 Q1 FORESTRY

Forests Pub Date : 2023-12-14 DOI:10.3390/f14122439

Jing Yang, Shengnan Xie, Dandan Du, Hongling Wei, Wenling Zhou, Ying Zhang, Zhonghua Tang, Dewen Li, Ying Liu

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引用次数: 0

摘要

研究了修剪处理对杜仲（Eucommia ulmoides Oliver）生长、光合作用特征和代谢产物的影响。实验于 2019 年 3 月至 8 月进行。采用了三种处理方法：不修剪树木（CK）、高度高于花盆上缘 20 厘米（T1）和高度高于花盆上缘 10 厘米（T2）。结果表明，与 CK 相比，修剪处理的枝条数量、叶片数量和茎干直径显著增加（p < 0.05）。同样，修剪处理的净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）、最大光合效率（Fv/Fm）和非光化学淬灭系数（NPQ）也明显增加（p < 0.05）。有趣的是，在 T2 中，Chl a、Chl b、Chl、Car 的含量以及 Chl a 含量与 Chl b 含量之间的比率分别明显增加（p < 0.05）。这些都验证了修剪处理能更好地促进 E. ulmoides 的植株生长。通过 KEGG 分析，GC-MS 分析表明，T2 处理鉴定出 36 种不同的初级代谢物，包括 11 种糖、13 种酸、5 种醇和 7 种其他化合物，其代谢物的相对含量高于 T1 处理，主要集中在四个主要的富集途径（半乳糖代谢、柠檬酸循环、乙醛酸和二羧酸代谢、淀粉和蔗糖代谢）。同时，相关分析表明，在 T2 处理中，D-半乳糖、D-甘露糖、琥珀酸的积累和光合色素含量与光合作用速率呈正相关（P < 0.05）。花盆上缘以上的修剪高度改变了溃疡苣苔初级代谢产物的积累，促进了植株再生能力。最后，通过超高效液相色谱（UPLC）分析，叶片中主要次生代谢物（玄皮素、玄皮苷、玄皮苷酸和松脂醇二葡萄糖苷）的产量在修剪处理中有所增加。这为定向生态栽培 E. ulmoides 提供了参考。

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Effect of Pruning Treatment on Growth Characteristics and Metabolites in Eucommia ulmoides Oliver (E. ulmoides)

The effect of pruning treatments on growth, photosynthesis characteristics, and metabolites were was studied in Eucommia ulmoides Oliver (E. ulmoides). The experiment was carried out from March–August 2019. Three treatments were used: non-pruned trees (CK), a height of 20 cm above the top edge of the flowerpot (T1), and a height of 10 cm above the top edge of the flowerpot (T2). The results showed that the branches branch number, leaves leaf number, and stem diameter increased significantly (p < 0.05) in pruning treatments compared with CK. Similarly, the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximum photosynthetic efficiency (Fv/Fm), and non-photochemical quenching coefficient (NPQ) increased significantly in pruning treatments (p < 0.05). Interestingly, the contents of Chl a, Chl b, Chl, Car, and the rate between the Chl a content and the Chl b content increased significantly (p < 0.05) in T2, respectively. These verified that it was a better way to enhance the plants growth of E. ulmoides for pruning treatments. The GC-MS analysis showed that 36 different primary metabolites were identified, including 11 sugars, 13 acids, 5 alcohols, and 7 other compounds, the relative content of their metabolites were was higher in the T2 treatment than that in the T1 treatment, which was mainly concentrated in four main enrichment pathways (Galactose metabolism; Citrate cycle; Glyoxylate and dicarboxylate metabolism; and starch and sucrose metabolism) via KEGG analysis. Meanwhile, correlation analysis showed there were was a positive correlation between the accumulation of D-Galactose, D-Mannose, Succinic acid, and photosynthetic pigment content, and the rate of photosynthesis in T2 treatment (p < 0.05). The pruning height above the top edge of the flowerpot changed the accumulation of primary metabolites and promoted plant regeneration ability in E. ulmoides. Finally, the yield of main secondary metabolites from leaves (Genipin, Geniposide, Geniposidic acid, and Pinoresinol diglucoside) were was increased in pruning treatments by UPLC analysis. It provided a reference for the directional ecological cultivation of E. ulmoides.

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来源期刊

Forests FORESTRY-

CiteScore

4.40

自引率

17.20%

发文量

1823

审稿时长

19.02 days

期刊介绍： Forests (ISSN 1999-4907) is an international and cross-disciplinary scholarly journal of forestry and forest ecology. It publishes research papers, short communications and review papers. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.