用点吸法检测培养液中的霍乱毒素

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
A. V. Boyko, E. A. Mikheeva, T. L. Zakharova, N. A. Osina
{"title":"用点吸法检测培养液中的霍乱毒素","authors":"A. V. Boyko,&nbsp;E. A. Mikheeva,&nbsp;T. L. Zakharova,&nbsp;N. A. Osina","doi":"10.1134/S0003683823080045","DOIUrl":null,"url":null,"abstract":"<div><p>An instrument-free method for detecting the ability of <i>Vibrio cholerae</i> strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.</p></div>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 8","pages":"1113 - 1117"},"PeriodicalIF":1.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks\",\"authors\":\"A. V. Boyko,&nbsp;E. A. Mikheeva,&nbsp;T. L. Zakharova,&nbsp;N. A. Osina\",\"doi\":\"10.1134/S0003683823080045\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An instrument-free method for detecting the ability of <i>Vibrio cholerae</i> strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.</p></div>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"59 8\",\"pages\":\"1113 - 1117\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683823080045\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823080045","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

我们开发了一种无需仪器的方法来检测霍乱弧菌菌株产生毒素的能力。该方法基于在浸渍棒上进行的点式酶联免疫吸附。浸渍棒所需的单克隆抗体比微孔板 ELISA 少 100 倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks

Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks

Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks

An instrument-free method for detecting the ability of Vibrio cholerae strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信