A. V. Boyko, E. A. Mikheeva, T. L. Zakharova, N. A. Osina
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Detection of Cholera Toxin in Culture Medium by Dot-Immunoassay on Dipsticks
An instrument-free method for detecting the ability of Vibrio cholerae strains to produce toxin has been developed. It is based on dot-ELISA performed on dipstiks. Dipsticks require 100 times less monoclonal antibodies than microplate ELISA.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.