胰腺癌分析揭示了 m6A 阅读器 IGF2BP2 在胰腺癌中的免疫作用和预后价值。

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Hui Deng , Hanming Yao , Shurui Zhou , Chong He , Yuzhou Huang , Yunlong Li , Hanwei Chen , Jianchang Shu
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引用次数: 0

摘要

简介胰腺导管腺癌(PDAC)是全球恶性程度最高的消化道肿瘤之一,预后不良,复发率高。PDAC 被认为是一种 "冷癌",免疫疗法对其无效。因此,为了改善 PDAC 患者的预后,迫切需要探索其对免疫疗法不敏感的机制:我们通过 TCGA 和 GETx 数据库对不同癌症患者的 IGF2BP 家族表达和存活率进行了胰腺癌分析。然后,我们确定了 IGF2BP2 在体外、体内和临床标本中的免疫学作用和预后价值:结果:在本研究中,我们发现 m6A 阅读器 IGF2BP2 是与胰腺癌临床相关性最大的 IGF2BP 家族成员。IGF2BP2 的高表达与 PDAC 的不良预后和免疫抑制微环境最为相关。通过敲除 IGF2BP2,我们发现肿瘤细胞的增殖和侵袭能力明显减弱。重要的是,我们发现 IGF2BP2 的表达与 PD-L1 等免疫抑制分子的高表达密切相关。IGF2BP2通过m6A甲基化控制调节PD-L1的mRNA稳定性,从而调节下游PD-L1的表达,我们在动物实验和人体组织标本中也得到了同样的验证:我们的研究为现有关于IGF2BP2调控的PD-L1信号通路作为胰腺癌潜在预后和免疫生物标志物的知识做出了贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pancancer analysis uncovers an immunological role and prognostic value of the m6A reader IGF2BP2 in pancreatic cancer

Introduction

Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant gastrointestinal tumors worldwide with a dismal prognosis and high relapse rate. PDAC is considered a “cold cancer” for which immunotherapy is not effective. Therefore, to improve the prognosis for PDAC patients, it is urgent to explore the mechanism driving its insensitivity to immunotherapy.

Materials and methods

We conducted pancancer analyses to test IGF2BP family expression and survival in patients with different cancers via TCGA and GETx databases. Then, we determined the immunological role and prognostic value of IGF2BP2 in vitro, in vivo and in clinical specimens.

Results

In the present study, we found that the m6A reader IGF2BP2 was the most clinically relevant member of the IGF2BP family for pancreatic cancer. High expression of IGF2BP2 was most associated with poor prognosis and an immunosuppressive microenvironment in PDAC. By IGF2BP2 knockdown, we found that tumor cell proliferation and invasive ability were significantly diminished. Importantly, we found that IGF2BP2 expression was closely associated with high expression of immunosuppressive molecules such as PD-L1. IGF2BP2 modulated downstream PD-L1 expression by regulating its mRNA stability via m6A methylation control, and we obtained the same verification in animal experiments and human tissue specimens.

Conclusion

Our study contributes to existing knowledge regarding the IGF2BP2-regulated PD-L1 signaling pathway as a potential prognostic and immune biomarker in pancreatic cancer.

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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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