{"title":"新鲜和冷冻干燥的槟榔叶、阔叶木薯叶和麝香草叶的抗糖尿病和抗氧化特性探究","authors":"Maheshika Peries , Seneveratne Navartne , Kanchana Abeysekara , Ranil Coorey , Amila Henagamage , Janaka Wijesinghe","doi":"10.1016/j.hermed.2023.100838","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>The drying method can have an impact on the stability and composition of bioactive compounds in plant materials, as well as their therapeutic activities. This study aimed at evaluating the anti-glycation and antioxidant properties of freeze-dried and fresh leaves of selected ayurvedic medicinal plants.</p></div><div><h3>Methods</h3><p>Fresh and freeze-dried leaves of <span><em>Hellenia speciosa, </em><em>Gymnema sylvestre</em></span> and <span><em>Coccinia grandis</em></span><span> were extracted separately with 95% ethanol. The extracts were studied for their anti-glucosidase, antiglycation, glycation-reversing activities and uptake of glucose by yeast cells<span><span><span>. Antioxidant activities of extracts were evaluated using </span>ABTS and </span>DPPH<span> free radical scavenging activities.</span></span></span></p></div><div><h3>Results</h3><p>All extracts of the freeze-dried leaves showed significant (<em>P</em> < 0.05) anti-glucosidase, antiglycation, and glycation-reversing activities than the fresh leaves of the same species. The freeze-dried leaf extract of <em>H. speciosa</em><span> demonstrated the highest antiglycation, anti-glucosidase, and glycation reversing activities among the plant species, with IC50 values of 100.76 ± 5.99 µg/ml, 28.11 ± 1.30 µg/ml and EC50 values of 147.24 ± 3.86 µg/ml, respectively. In addition to that, freeze-dried </span><em>H. speciosa</em> showed the maximum ABTS and DPPH free radical scavenging activity.</p></div><div><h3>Conclusions</h3><p>This study confirmed that freeze-dried leaves have promising anti-diabetic and antioxidant properties that can be used to develop herbal nutritional supplements<span><span> or functional foods for diabetes mellitus </span>treatment.</span></p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploration of antidiabetic and antioxidant properties of fresh and freeze-dried Hellenia speciosa, Coccinia grandis and Gymnema sylvestre leaves\",\"authors\":\"Maheshika Peries , Seneveratne Navartne , Kanchana Abeysekara , Ranil Coorey , Amila Henagamage , Janaka Wijesinghe\",\"doi\":\"10.1016/j.hermed.2023.100838\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>The drying method can have an impact on the stability and composition of bioactive compounds in plant materials, as well as their therapeutic activities. This study aimed at evaluating the anti-glycation and antioxidant properties of freeze-dried and fresh leaves of selected ayurvedic medicinal plants.</p></div><div><h3>Methods</h3><p>Fresh and freeze-dried leaves of <span><em>Hellenia speciosa, </em><em>Gymnema sylvestre</em></span> and <span><em>Coccinia grandis</em></span><span> were extracted separately with 95% ethanol. The extracts were studied for their anti-glucosidase, antiglycation, glycation-reversing activities and uptake of glucose by yeast cells<span><span><span>. Antioxidant activities of extracts were evaluated using </span>ABTS and </span>DPPH<span> free radical scavenging activities.</span></span></span></p></div><div><h3>Results</h3><p>All extracts of the freeze-dried leaves showed significant (<em>P</em> < 0.05) anti-glucosidase, antiglycation, and glycation-reversing activities than the fresh leaves of the same species. The freeze-dried leaf extract of <em>H. speciosa</em><span> demonstrated the highest antiglycation, anti-glucosidase, and glycation reversing activities among the plant species, with IC50 values of 100.76 ± 5.99 µg/ml, 28.11 ± 1.30 µg/ml and EC50 values of 147.24 ± 3.86 µg/ml, respectively. In addition to that, freeze-dried </span><em>H. speciosa</em> showed the maximum ABTS and DPPH free radical scavenging activity.</p></div><div><h3>Conclusions</h3><p>This study confirmed that freeze-dried leaves have promising anti-diabetic and antioxidant properties that can be used to develop herbal nutritional supplements<span><span> or functional foods for diabetes mellitus </span>treatment.</span></p></div>\",\"PeriodicalId\":56077,\"journal\":{\"name\":\"Journal of Herbal Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbal Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210803323002166\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210803323002166","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Exploration of antidiabetic and antioxidant properties of fresh and freeze-dried Hellenia speciosa, Coccinia grandis and Gymnema sylvestre leaves
Introduction
The drying method can have an impact on the stability and composition of bioactive compounds in plant materials, as well as their therapeutic activities. This study aimed at evaluating the anti-glycation and antioxidant properties of freeze-dried and fresh leaves of selected ayurvedic medicinal plants.
Methods
Fresh and freeze-dried leaves of Hellenia speciosa, Gymnema sylvestre and Coccinia grandis were extracted separately with 95% ethanol. The extracts were studied for their anti-glucosidase, antiglycation, glycation-reversing activities and uptake of glucose by yeast cells. Antioxidant activities of extracts were evaluated using ABTS and DPPH free radical scavenging activities.
Results
All extracts of the freeze-dried leaves showed significant (P < 0.05) anti-glucosidase, antiglycation, and glycation-reversing activities than the fresh leaves of the same species. The freeze-dried leaf extract of H. speciosa demonstrated the highest antiglycation, anti-glucosidase, and glycation reversing activities among the plant species, with IC50 values of 100.76 ± 5.99 µg/ml, 28.11 ± 1.30 µg/ml and EC50 values of 147.24 ± 3.86 µg/ml, respectively. In addition to that, freeze-dried H. speciosa showed the maximum ABTS and DPPH free radical scavenging activity.
Conclusions
This study confirmed that freeze-dried leaves have promising anti-diabetic and antioxidant properties that can be used to develop herbal nutritional supplements or functional foods for diabetes mellitus treatment.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.