{"title":"条纹草水醇提取物对人脂肪间充质干细胞（hADSCs）分化为软骨细胞和骨细胞的影响","authors":"Zahra Bagheri-Hosseinabadi , Fatemeh Javani Jouni , Jaber Zafari , Saba Sadeghi , Mitra Abbasifard","doi":"10.1016/j.hermed.2023.100841","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p><span><span>Osteoarthritis is a major type of </span>joint disease. Cell therapy is the main hopeful therapeutic method to treat this disorder. The aim of this study was to determine the effect of </span><span><em>Scrophularia</em><em> striata</em></span><span> hydroalcoholic extract on the differentiation of human adipose tissue-derived stem cells to chondrogenic and osteogenic cells a perspective for treatment of Osteoarthritis.</span></p></div><div><h3>Methods</h3><p>The hydroalcoholic extract of <em>S. striata</em> were prepared. The <em>S. striata</em><span><span> extract was standardised according to gallic acid<span> and querctin by HPLC<span><span>. Human adipose-derived mesenchymal stem cells (hADSCs) were cultured in DMEM/F12 complemented with 10% fetal bovine serum. After the identification of cells, </span>cell viability was measured by </span></span></span>MTT assays, 24 and 48 hours after treatment with different concentrations of plant extract. The expression pattern of special chondrogenic and osteogenic genes and stem cell markers was evaluated through real time-PCR.</span></p></div><div><h3>Results</h3><p>Our findings showed that the <em>S. striata</em> plant extract has the capability of reducing the viability of hADSCs cells at concentration of 800 and 1000 ug/ml after 24 and 48 hours. The dosage of <em>S. striata</em> that significantly induced hADSCs cell differentiation is IC25, and the IC50 for hADSCs was 865.0 and 642.9 µg/ml after 24 and 48 hours, respectively. The hydroalcoholic extract of the <em>S. striata</em><span> was standardised according to Gallic acid (0.33 mg gallic acid/g Extract) and Quercetin (2.81 mg quercetin/g Extract) compounds.</span></p></div><div><h3>Conclusion</h3><p>The results showed that in addition to exerting anti-inflammatory effects, <em>S. striata</em> can induce the orientation of hADSCs cells into osteogenic and chondrogenic cells.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Scrophularia striata hydroalcoholic extract on differentiation of human adipose-derived mesenchymal stem cells into chondrocytes and osteocytes\",\"authors\":\"Zahra Bagheri-Hosseinabadi , Fatemeh Javani Jouni , Jaber Zafari , Saba Sadeghi , Mitra Abbasifard\",\"doi\":\"10.1016/j.hermed.2023.100841\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p><span><span>Osteoarthritis is a major type of </span>joint disease. Cell therapy is the main hopeful therapeutic method to treat this disorder. The aim of this study was to determine the effect of </span><span><em>Scrophularia</em><em> striata</em></span><span> hydroalcoholic extract on the differentiation of human adipose tissue-derived stem cells to chondrogenic and osteogenic cells a perspective for treatment of Osteoarthritis.</span></p></div><div><h3>Methods</h3><p>The hydroalcoholic extract of <em>S. striata</em> were prepared. The <em>S. striata</em><span><span> extract was standardised according to gallic acid<span> and querctin by HPLC<span><span>. Human adipose-derived mesenchymal stem cells (hADSCs) were cultured in DMEM/F12 complemented with 10% fetal bovine serum. After the identification of cells, </span>cell viability was measured by </span></span></span>MTT assays, 24 and 48 hours after treatment with different concentrations of plant extract. The expression pattern of special chondrogenic and osteogenic genes and stem cell markers was evaluated through real time-PCR.</span></p></div><div><h3>Results</h3><p>Our findings showed that the <em>S. striata</em> plant extract has the capability of reducing the viability of hADSCs cells at concentration of 800 and 1000 ug/ml after 24 and 48 hours. The dosage of <em>S. striata</em> that significantly induced hADSCs cell differentiation is IC25, and the IC50 for hADSCs was 865.0 and 642.9 µg/ml after 24 and 48 hours, respectively. The hydroalcoholic extract of the <em>S. striata</em><span> was standardised according to Gallic acid (0.33 mg gallic acid/g Extract) and Quercetin (2.81 mg quercetin/g Extract) compounds.</span></p></div><div><h3>Conclusion</h3><p>The results showed that in addition to exerting anti-inflammatory effects, <em>S. striata</em> can induce the orientation of hADSCs cells into osteogenic and chondrogenic cells.</p></div>\",\"PeriodicalId\":56077,\"journal\":{\"name\":\"Journal of Herbal Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbal Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210803323002191\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210803323002191","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Effect of Scrophularia striata hydroalcoholic extract on differentiation of human adipose-derived mesenchymal stem cells into chondrocytes and osteocytes
Introduction
Osteoarthritis is a major type of joint disease. Cell therapy is the main hopeful therapeutic method to treat this disorder. The aim of this study was to determine the effect of Scrophularia striata hydroalcoholic extract on the differentiation of human adipose tissue-derived stem cells to chondrogenic and osteogenic cells a perspective for treatment of Osteoarthritis.
Methods
The hydroalcoholic extract of S. striata were prepared. The S. striata extract was standardised according to gallic acid and querctin by HPLC. Human adipose-derived mesenchymal stem cells (hADSCs) were cultured in DMEM/F12 complemented with 10% fetal bovine serum. After the identification of cells, cell viability was measured by MTT assays, 24 and 48 hours after treatment with different concentrations of plant extract. The expression pattern of special chondrogenic and osteogenic genes and stem cell markers was evaluated through real time-PCR.
Results
Our findings showed that the S. striata plant extract has the capability of reducing the viability of hADSCs cells at concentration of 800 and 1000 ug/ml after 24 and 48 hours. The dosage of S. striata that significantly induced hADSCs cell differentiation is IC25, and the IC50 for hADSCs was 865.0 and 642.9 µg/ml after 24 and 48 hours, respectively. The hydroalcoholic extract of the S. striata was standardised according to Gallic acid (0.33 mg gallic acid/g Extract) and Quercetin (2.81 mg quercetin/g Extract) compounds.
Conclusion
The results showed that in addition to exerting anti-inflammatory effects, S. striata can induce the orientation of hADSCs cells into osteogenic and chondrogenic cells.
期刊介绍：
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.
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