丝兰绿色纳米技术--植物化学物质--功能化金纳米粒子--对前列腺癌和乳腺癌的抗肿瘤功效

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY
Nanotechnology, Science and Applications Pub Date : 2023-12-11 eCollection Date: 2023-01-01 DOI:10.2147/NSA.S437812
Velaphi C Thipe, Ananya Jatar, Alice Raphael Karikachery, Kavita K Katti, Kattesh V Katti
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引用次数: 0

摘要

目的:我们报告了一种创新的绿色纳米技术,利用丝兰富含电子的植物化学物质鸡尾酒,在不使用任何外部化学还原剂的情况下合成生物相容性金纳米粒子,并评估其抗癌活性:方法:制备含有鸡尾酒植物化学物质的丝兰提取物,并将其用于将金盐转化为丝兰植物化学物质包裹的金纳米粒子(YF-AuNPs)。此外,还制备了阿拉伯树胶稳定的 YF-AuNPs (GAYF-AuNPs),以提高体外/体内稳定性。对前列腺癌(PC-3)和乳腺癌(MDAMB-231)细胞系的抗癌活性进行了评估。使用促肿瘤巨噬细胞系测试了金纳米粒子的靶向能力:结果:新型纳米药物YF-AuNPs和GAYF-AuNPs的综合表征显示,金纳米粒子呈球形,单分散,zeta电位适中(分别为-19和-20 mV),表明其在体外/体内均稳定。YF-AuNPs 的核心尺寸(14 ± 5 nm)和 GAYF-AuNPs 的核心尺寸(10 ± 5 nm)适合通过增强渗透性和滞留(EPR)效应以及受体介导的内吞作用最佳地渗透到肿瘤细胞中。值得注意的是,YF-AuNPs 通过诱导早期和晚期细胞凋亡,对前列腺肿瘤(PC-3)和乳腺肿瘤(MDAMB-231)具有很强的抗癌活性。此外,YF-AuNPs 还提高了抗肿瘤细胞因子(TNF-α 和 IL-12)的水平,降低了促肿瘤细胞因子(IL-6 和 IL-10)的水平,为 YF-AuNPs 的免疫调节特性提供了令人信服的证据:总之,这些Y. filamentosa植物化学物质功能化纳米阿育吠陀药剂对癌细胞具有选择性毒性,而对正常细胞则无影响。最值得注意的是,据我们所知,这是首次有研究表明 YF-AuNP 对促癌巨噬细胞系有靶向疗效,从而为癌症治疗提供了一种免疫调节途径。这项研究通过选择性疗效和免疫调节潜力,为草药和纳米阿育吠陀疗法治疗人类癌症开辟了一条新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Green Nanotechnology of Yucca filamentosa- Phytochemicals-Functionalized Gold Nanoparticles-Antitumor Efficacy Against Prostate and Breast Cancers.

Purpose: We report an innovative green nanotechnology utilizing an electron-rich cocktail of phytochemicals from Yucca filamentosa L. to synthesize biocompatible gold nanoparticles without the use of any external chemical reducing agents and evaluate their anti-cancer activity.

Methods: Yucca filamentosa L. extract, containing a cocktail of phytochemicals, was prepared, and used to transform gold salt into Y. filamentosa phytochemicals encapsulated gold nanoparticles (YF-AuNPs). Additionally, gum arabic stabilized YF-AuNPs (GAYF-AuNPs) were also prepared to enhance the in vitro/in vivo stability. Anticancer activity was evaluated against prostate (PC-3) and breast (MDAMB-231) cancer cell lines. Targeting abilities of gold nanoparticles were tested using pro-tumor macrophage cell lines.

Results: Comprehensive characterization of new nanomedicine agents YF-AuNPs and GAYF-AuNPs revealed spherical, and monodisperse AuNPs with moderate zeta potentials (-19 and -20 mV, respectively), indicating in vitro/in vivo stability. The core size of YF-AuNPs (14 ± 5 nm) and GAYF-AuNPs (10 ± 5 nm) is suitable for optimal penetration into tumor cells through both enhanced permeability and retention (EPR) effect as well as through the receptor mediated endocytosis. Notably, YF-AuNPs exhibited potent anticancer activity against prostate (PC-3) and breast tumors (MDAMB-231) by inducing early and late apoptotic stages. Moreover, YF-AuNPs resulted in elevated levels of anti-tumor cytokines (TNF-α and IL-12) and reduced levels of pro-tumor cytokines (IL-6 and IL-10), provide compelling evidence on the immunomodulatory property of YF-AuNPs.

Conclusion: Overall, these Y. filamentosa phytochemicals functionalized nano-Ayurvedic medicine agents demonstrated selective toxicity to cancer cells while sparing normal cells. Most notably, to our knowledge, this is the first study that shows YF-AuNP's targeting efficacy toward pro-tumor macrophage cell lines, suggesting an immunomodulatory pathway for cancer treatment. This work introduces a novel avenue for herbal and nano-Ayurvedic approaches to human cancer treatment, mediated through selective efficacy and immunomodulatory potential.

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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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