小檗碱可减少 S100B 的生成,从而调节烧伤小鼠肠道血管屏障的通透性。

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Pharmaceutical Biology Pub Date : 2024-12-01 Epub Date: 2023-12-18 DOI:10.1080/13880209.2023.2291679
Aiwen Feng, Shaosheng Su, Cheng Li, Yutian Kang, Jiasheng Qiu, Jun Zhou
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引用次数: 0

摘要

背景:小檗碱(BBR)可调节肠胶质细胞(EGCs)和肠道血管屏障(GVB):探讨小檗碱是否通过 S100B 通路调节 GVB 的通透性:通过烧伤或S100B灌肠诱导C57BL/6J小鼠的GVB高通透性。烧伤前灌胃 BBR(25 或 50 mg/kg/d,3 d)。烧伤后静脉注射 S100B 单克隆抗体(S100BmAb)。用 S100B、S100B 加 BBR 或 Z-IETD-FMK 处理小鼠肠微血管内皮细胞(MIMECs)。用 FITC-右旋糖酐检测 GVB 的通透性,用 ELISA 检测 S100B,用免疫印迹检测 caspase-8、β-catenin、occludin 和 PV-1:烧伤后 36 小时,血清和结肠粘膜中的 S100B 升高至峰值(分别为 147.00 ± 4.95 ng/mL 和 160.30 ± 8.50 ng/mg),但 BBR 可降低烧伤诱导的血清(126.20 ± 6.30 或 90.60 ± 3.78 ng/mL)和粘膜(125.80 ± 12.40 或 91.20 ± 8.54 ng/mg)中的 S100B。烧伤后 48 小时,烧伤提高了 GVB 的通透性(血清 FITC-dextran 111.40 ± 8.56 pg/mL),但 BBR 降低了 GVB 的通透性(血清 FITC-dextran 89.20 ± 6.98 或 68.60 ± 5.50 ng/mL)。S100B 灌肠剂(1 μM)会加重烧伤后升高的 GVB 通透性(142.80 ± 8.07 pg/mL)和 PV-1,但 BBR 可拮抗 S100B 的作用。Z-IETD-FMK (5 μM)增加了 S100B 诱导的 FITC-葡聚糖通透性(205.80 ± 9.70 至 263.80 ± 11.04 AUs),同时降低了 MIMECs 中的β-catenin。BBR(5 μM)降低了 S100B 诱导的通透性(104.20 ± 9.65 AUs),并增加了 Caspase-8、β-catenin 和 occludin:BBR通过调节S100B/caspase-8/β-catenin通路降低烧伤诱导的GVB高通透性,可能与EGCs有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Berberine decreases S100B generation to regulate gut vascular barrier permeability in mice with burn injury.

Context: Berberine (BBR) can regulate enteric glial cells (EGCs) and the gut vascular barrier (GVB).Objective: To explore whether BBR regulates GVB permeability via the S100B pathway.Materials and methods: GVB hyperpermeability in C57BL/6J mice was induced by burns or S100B enema. BBR (25 or 50 mg/kg/d, 3 d) was gavaged preburn. S100B monoclonal antibody (S100BmAb) was i.v. injected postburn. Mouse intestinal microvascular endothelial cells (MIMECs) were treated with S100B, S100B plus BBR, or Z-IETD-FMK. GVB permeability was assayed by FITC-dextran, S100B by ELISA, caspase-8, β-catenin, occludin and PV-1 by immunoblot.Results: Burns elevated S100B in serum and in colonic mucosa to a peak (147.00 ± 4.95 ng/mL and 160.30 ± 8.50 ng/mg, respectively) at 36 h postburn, but BBR decreased burns-induced S100B in serum (126.20 ± 6.30 or 90.60 ± 3.78 ng/mL) and in mucosa (125.80 ± 12.40 or 91.20 ± 8.54 ng/mg). Burns raised GVB permeability (serum FITC-dextran 111.40 ± 8.56 pg/mL) at 48 h postburn, but BBR reduced GVB permeability (serum FITC-dextran 89.20 ± 6.98 or 68.60 ± 5.50 ng/mL). S100B enema (1 μM) aggravated burns-raised GVB permeability (142.80 ± 8.07 pg/mL) and PV-1, but the effect of S100B was antagonized by BBR. Z-IETD-FMK (5 μM) increased S100B-induced permeability to FITC-dextran (205.80 ± 9.70 to 263.80 ± 11.04 AUs) while reducing β-catenin in MIMECs. BBR (5 μM) reduced S100B-induced permeability (104.20 ± 9.65 AUs) and increased caspase-8, β-catenin and occludin.Discussion and conclusion: BBR decreases burns-induced GVB hyperpermeability via modulating S100B/caspase-8/β-catenin pathway and may involve EGCs.

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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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