传统索氏香蒲叶提取物对斯普拉格-道利大鼠的急性和亚慢性毒性评估

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2023-12-11 eCollection Date: 2023-01-01 DOI:10.1155/2023/8575741
Jacob Apibilla Ayembilla, Abdul Raouf Khalid, Sharif Buari Abubakari, Abdul Rashid Adams, Felix Abekah Botchway, Stephen Antwi, Phyllis Naa Yarley Otu, Michael Appiah, George Osei-Adjei, Kwame Owen Kottoh, Peace Ahiabenu-Williams, Olga Quasie
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引用次数: 0

摘要

背景:在加纳,柠檬香蒲叶与番石榴、掌叶和石灰一起加工成煎剂,用于治疗发烧。为鼓励使用这种植物,需要对其安全性进行临床前验证。本研究调查了传统索氏乙醇香蒲叶提取物对斯普拉格-道利大鼠的急性和亚慢性毒性:采用传统索氏提取法(CSE)用98%的乙醇萃取粉碎的香蒲叶,然后干燥。在急性毒性研究中,给 6 只雌性 Sprague-Dawley 大鼠注射单剂量 5000 毫克/千克体重,给对照组(6 只)注射 1 毫升/100 克体重的生理盐水。在亚慢性研究中,处理组分别施用 200 毫克/千克、600 毫克/千克和 1200 毫克/千克的 CSE C. citratus 叶提取物,为期六周。对大鼠的血液、尿液和血清样本进行了分析。研究结束时对大鼠的肝、心、肾、脾和肺进行了组织病理学检查。进行方差分析(ANOVA),以确定试验大鼠与对照大鼠之间的统计学差异(P < 0.05):结果显示,在治疗期间,对照组大鼠和试验组大鼠的尿液分析和血液分析没有明显的统计学差异(P > 0.05)。同样,CSE C. citratus 叶提取物也没有诱导处理组大鼠发生任何明显的生化变化;不过,处理组大鼠的体重有所下降。与对照组相比,受试大鼠的心、肝、脾、肺和肾没有明显的形态变化:结论:CSE 柠檬叶乙醇提取物具有减轻体重的作用,长期服用该提取物可能不会对消费者造成任何器官特异性毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Acute and Subchronic Toxicity Assessment of Conventional Soxhlet Cymbopogon citratus Leaves Extracts in Sprague-Dawley Rats.

Background: In Ghana, Cymbopogon citratus leaves together with guava, pawpaw, and lime are processed into a decoction to treat fever. To encourage its usage, preclinical validation of the safety profile of the plant is required. The acute and subchronic toxicities of the conventional Soxhlet ethanolic Cymbopogon citratus leaves extract in Sprague-Dawley rats were investigated.

Methods: Pulverized Cymbopogon citratus leaves were extracted with 98% ethanol using the conventional Soxhlet extraction (CSE) method and dried. In the acute toxicity study, a single dose of 5000 mg/kg body weight was administered to six female Sprague-Dawley rats and 1 ml/100 g body weight normal saline to control (6) once, and signs of toxicity were observed every hour for the first 12 hr, 24 hr, and 48 hr through to 14 days. In the subchronic study, the treatment groups were administered 200 mg/kg, 600 mg/kg, and 1200 mg/kg, respectively, of the CSE C. citratus leaves extract for six weeks. Analyses were conducted on the blood, urine, and serum samples of the rats. Histopathological examination of the liver, heart, kidney, spleen, and lungs was carried out at termination. Analysis of variance (ANOVA) was performed to determine statistically significant differences between the test and control rats at P  <  0.05.

Results: The results revealed that there were no statistically significant differences (p  >  0.05) in the urinalysis and haematological analysis between control and test rats over the treatment period. Similarly, CSE C. citratus leaves extract did not induce any significant biochemical changes in the treatment group; however, there was a weight loss effect on the treated rats. There were no noticeable morphological changes in the heart, liver, spleen, lung, and kidney of the test rats compared to the control.

Conclusion: CSE ethanolic C. citratus leaves extract has a weight loss effect, and long-term administration of the extract may not cause any organ-specific toxicity to the consumers.

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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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