Dendrobium Officinale Kinura et Migo 糖蛋白通过调节细胞外基质分泌和增殖期成纤维细胞增殖促进皮肤伤口愈合。

IF 3.8 3区 医学 Q2 CELL BIOLOGY
Wound Repair and Regeneration Pub Date : 2024-01-01 Epub Date: 2023-12-29 DOI:10.1111/wrr.13144
Jia Li, Qian Zhao, Xiaoyu Gao, Tianyi Dai, Zilin Bai, Jun Sheng, Yang Tian, Zhongbin Bai
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引用次数: 0

摘要

铁皮石斛(Dendrobium officinale Kinura et Migo,DOKM)具有多种药用价值,但其促进伤口愈合的能力此前尚未见报道。本研究的目的是调查 DOKM 糖蛋白(DOKMG)对大鼠伤口愈合作用的增殖阶段,并阐明其体外作用机制。本研究将含有 DOKMG 的混合软膏涂抹在全厚皮肤切除大鼠模型的背侧皮肤伤口上,结果表明增殖期的伤口愈合速度比凡士林快。组织学分析表明,DOKMG 能促进伤口皮肤的再上皮化。免疫荧光染色和定量聚合酶链式反应(q-PCR)检测表明,在肉芽组织形成期间,DOKMG 能促进纤连蛋白的分泌,抑制胶原蛋白 IV 的分泌,这表明 DOKMG 能通过精确调节细胞外基质(ECM)的分泌来加速肉芽组织的形成。此外,我们还证明了 DOKMG 可通过调节 ECM 的分泌来增强二维创伤中成纤维细胞(3 T6 细胞)的迁移和增殖,其机制可能与表皮生长因子受体(EGFR)信号传导控制下的 AKT 和 JAK/STAT 通路有关。总之,我们已经证明 DOKMG 能在增殖期促进伤口愈合。因此,我们认为 DOKMG 有可能应用于皮肤伤口的治疗和管理。本文受版权保护。保留所有权利。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dendrobium officinale Kinura et Migo glycoprotein promotes skin wound healing by regulating extracellular matrix secretion and fibroblast proliferation on the proliferation phase.

Dendrobium officinale Kinura et Migo (DOKM) has a variety of medicinal applications; however, its ability to promote wound healing has not been previously reported. The purpose of this study is to investigate the proliferative phase of the wound-healing effect of DOKM glycoprotein (DOKMG) in rats and to elucidate its mechanism of action in vitro. In the present study, the ointment mixture containing DOKMG was applied to the dorsal skin wounds of the full-thickness skin excision rat model, and the results showed that the wound healing speed was faster in the proliferative phase than vaseline. Histological analysis demonstrates that DOKMG promoted the re-epithelialization of wound skin. Immunofluorescence staining and quantitative polymerase chain reaction assays revealed that DOKMG promotes the secretion of Fibronectin and inhibits the secretion of Collagen IV during the granulation tissue formation period, indicating that DOKMG could accelerate the formation of granulation tissue by precisely regulating extracellular matrix (ECM) secretion. In addition, we demonstrated that DOKMG enhanced the migration and proliferation of fibroblast (3T6 cell) in two-dimensional trauma by regulating the secretion of ECM, via a mechanism that may implicate the AKT and JAK/STAT pathways under the control of epidermal growth factor receptor (EGFR) signalling. In summary, we have demonstrated that DOKMG promotes wound healing during the proliferative phase. Therefore, we suggest that DOKMG may have a potential therapeutic application for the treatment and management of cutaneous wounds.

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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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