基于部分全身照射后人体外周血淋巴细胞中伽马-H2AX定量的生物测定法

IF 1 4区 医学 Q4 ENVIRONMENTAL SCIENCES
Health physics Pub Date : 2024-03-01 Epub Date: 2023-12-13 DOI:10.1097/HP.0000000000001779
Li-Ping Ma, Jie Chen, Meng-Meng Liu, Juan Yan, Jia-Qi Xiang, Mei Tian, Ling Gao, Qing-Jie Liu
{"title":"基于部分全身照射后人体外周血淋巴细胞中伽马-H2AX定量的生物测定法","authors":"Li-Ping Ma, Jie Chen, Meng-Meng Liu, Juan Yan, Jia-Qi Xiang, Mei Tian, Ling Gao, Qing-Jie Liu","doi":"10.1097/HP.0000000000001779","DOIUrl":null,"url":null,"abstract":"<p><strong>Abstract: </strong>Quantification of gamma-H2AX foci can estimate exposure to ionizing radiation. Most nuclear and radiation accidents are partial-body irradiation, and the doses estimated using the total-body irradiation dose estimation formula are often lower than the actual dose. To evaluate the dose-response relation of gamma-H2AX foci in human peripheral blood lymphocytes after partial-body irradiation and establish a simple and high throughput model to estimate partial-body irradiation dose, we collected human peripheral blood and irradiated with 0-, 0.5-, 1-, 2-, 3-, 4-, 5-, 6-, and 8-Gy gamma rays to simulate total-body irradiation in vitro. Gamma-H2AX foci were quantitated by flow cytometry at 1 h after irradiation, and a dose-response curve was established for total-body irradiation dose estimation. Then, a partial-body irradiation dose-response calibration curve was established by adding calibration coefficients based on the Dolphin method. To reflect the data distribution of all doses more realistically, the partial-body irradiation dose-response calibration curve was divided into two sections. In addition, partial-body irradiation was simulated in vitro, and the PBI data were substituted into curves to verify the accuracy of the two partial-body irradiation calibration curves. Results showed that the dose estimation variations were all less than 30% except the 25% partial-body irradiation group at 1 Gy, and the partial-body irradiation calibration dose-response curves were YF 1 = - 3.444 x 2 + 18.532 x + 3.109, R 2 = 0.92 (YF ≤ 27.95); YF 2 = - 2.704 x 2 + 37.97 x - 56.45, R 2 = 0.86 (YF > 27.95). Results also suggested that the partial-body irradiation dose-response calibration curve based on the gamma-H2AX foci quantification in human peripheral blood lymphocytes is a simple and high throughput model to assess partial-body irradiation dose.</p>","PeriodicalId":12976,"journal":{"name":"Health physics","volume":" ","pages":"134-140"},"PeriodicalIF":1.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biodosimetry Based on Gamma-H2AX Quantification in Human Peripheral Blood Lymphocytes after Partial-body Irradiation.\",\"authors\":\"Li-Ping Ma, Jie Chen, Meng-Meng Liu, Juan Yan, Jia-Qi Xiang, Mei Tian, Ling Gao, Qing-Jie Liu\",\"doi\":\"10.1097/HP.0000000000001779\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Abstract: </strong>Quantification of gamma-H2AX foci can estimate exposure to ionizing radiation. Most nuclear and radiation accidents are partial-body irradiation, and the doses estimated using the total-body irradiation dose estimation formula are often lower than the actual dose. To evaluate the dose-response relation of gamma-H2AX foci in human peripheral blood lymphocytes after partial-body irradiation and establish a simple and high throughput model to estimate partial-body irradiation dose, we collected human peripheral blood and irradiated with 0-, 0.5-, 1-, 2-, 3-, 4-, 5-, 6-, and 8-Gy gamma rays to simulate total-body irradiation in vitro. Gamma-H2AX foci were quantitated by flow cytometry at 1 h after irradiation, and a dose-response curve was established for total-body irradiation dose estimation. Then, a partial-body irradiation dose-response calibration curve was established by adding calibration coefficients based on the Dolphin method. To reflect the data distribution of all doses more realistically, the partial-body irradiation dose-response calibration curve was divided into two sections. In addition, partial-body irradiation was simulated in vitro, and the PBI data were substituted into curves to verify the accuracy of the two partial-body irradiation calibration curves. Results showed that the dose estimation variations were all less than 30% except the 25% partial-body irradiation group at 1 Gy, and the partial-body irradiation calibration dose-response curves were YF 1 = - 3.444 x 2 + 18.532 x + 3.109, R 2 = 0.92 (YF ≤ 27.95); YF 2 = - 2.704 x 2 + 37.97 x - 56.45, R 2 = 0.86 (YF > 27.95). Results also suggested that the partial-body irradiation dose-response calibration curve based on the gamma-H2AX foci quantification in human peripheral blood lymphocytes is a simple and high throughput model to assess partial-body irradiation dose.</p>\",\"PeriodicalId\":12976,\"journal\":{\"name\":\"Health physics\",\"volume\":\" \",\"pages\":\"134-140\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Health physics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1097/HP.0000000000001779\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Health physics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/HP.0000000000001779","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/13 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

摘要:伽马-H2AX 病灶的定量可以估算电离辐射照射量。大多数核事故和辐射事故都是局部辐照,用全身辐照剂量估算公式估算的剂量往往低于实际剂量。为了评估人体部分辐照后外周血淋巴细胞中伽马-H2AX灶的剂量-反应关系,并建立一个简单、高通量的模型来估算部分辐照剂量,我们采集了人体外周血,并用0、0.5、1、2、3、4、5、6、8-Gy伽马射线进行体外模拟全身辐照。辐照后1小时,用流式细胞术对伽马-H2AX病灶进行定量,并建立了剂量-反应曲线,用于估算全身辐照剂量。然后,通过添加基于 Dolphin 法的校准系数,建立了部分体照射剂量-反应校准曲线。为了更真实地反映所有剂量的数据分布,部分体辐照剂量-反应校准曲线被分为两段。此外,还在体外模拟了部分体照射,并将 PBI 数据代入曲线,以验证两条部分体照射校准曲线的准确性。结果表明,除1 Gy的25%部分体照射组外,其他组的剂量估计变化均小于30%,部分体照射校准剂量-反应曲线为YF1 = - 3.444 x 2 + 18.532 x + 3.109,R2 = 0.92(YF ≤ 27.95);YF2 = - 2.704 x 2 + 37.97 x - 56.45,R2 = 0.86(YF > 27.95)。结果还表明,基于人体外周血淋巴细胞中γ-H2AX病灶定量的部分全身辐照剂量-反应校准曲线是一种简单、高通量的评估部分全身辐照剂量的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biodosimetry Based on Gamma-H2AX Quantification in Human Peripheral Blood Lymphocytes after Partial-body Irradiation.

Abstract: Quantification of gamma-H2AX foci can estimate exposure to ionizing radiation. Most nuclear and radiation accidents are partial-body irradiation, and the doses estimated using the total-body irradiation dose estimation formula are often lower than the actual dose. To evaluate the dose-response relation of gamma-H2AX foci in human peripheral blood lymphocytes after partial-body irradiation and establish a simple and high throughput model to estimate partial-body irradiation dose, we collected human peripheral blood and irradiated with 0-, 0.5-, 1-, 2-, 3-, 4-, 5-, 6-, and 8-Gy gamma rays to simulate total-body irradiation in vitro. Gamma-H2AX foci were quantitated by flow cytometry at 1 h after irradiation, and a dose-response curve was established for total-body irradiation dose estimation. Then, a partial-body irradiation dose-response calibration curve was established by adding calibration coefficients based on the Dolphin method. To reflect the data distribution of all doses more realistically, the partial-body irradiation dose-response calibration curve was divided into two sections. In addition, partial-body irradiation was simulated in vitro, and the PBI data were substituted into curves to verify the accuracy of the two partial-body irradiation calibration curves. Results showed that the dose estimation variations were all less than 30% except the 25% partial-body irradiation group at 1 Gy, and the partial-body irradiation calibration dose-response curves were YF 1 = - 3.444 x 2 + 18.532 x + 3.109, R 2 = 0.92 (YF ≤ 27.95); YF 2 = - 2.704 x 2 + 37.97 x - 56.45, R 2 = 0.86 (YF > 27.95). Results also suggested that the partial-body irradiation dose-response calibration curve based on the gamma-H2AX foci quantification in human peripheral blood lymphocytes is a simple and high throughput model to assess partial-body irradiation dose.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Health physics
Health physics 医学-公共卫生、环境卫生与职业卫生
CiteScore
4.20
自引率
0.00%
发文量
324
审稿时长
3-8 weeks
期刊介绍: Health Physics, first published in 1958, provides the latest research to a wide variety of radiation safety professionals including health physicists, nuclear chemists, medical physicists, and radiation safety officers with interests in nuclear and radiation science. The Journal allows professionals in these and other disciplines in science and engineering to stay on the cutting edge of scientific and technological advances in the field of radiation safety. The Journal publishes original papers, technical notes, articles on advances in practical applications, editorials, and correspondence. Journal articles report on the latest findings in theoretical, practical, and applied disciplines of epidemiology and radiation effects, radiation biology and radiation science, radiation ecology, and related fields.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信