Nrf2提高了脂肪来源干细胞在大鼠神经源性勃起功能障碍模型中的治疗效率。

IF 2.4 3区 医学 Q2 ANDROLOGY
Shangbin Yang, Wancheng Shi, Qianhui Liu, Yingqiu Song, Jiafeng Fang
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引用次数: 0

摘要

背景:术中神经损伤导致的勃起功能障碍(ED)是盆腔手术的主要并发症。脂肪源性干细胞(ADSCs)在双侧海绵体神经损伤(BCNI)大鼠模型中具有治疗潜力,但体内存活率不足在很大程度上限制了其应用。核因子-E2相关因子(Nrf2)是调节细胞抗氧化压力的关键转录因子。在这项工作中,我们研究了 Nrf2 表达调控对 ADSCs 存活率的影响,并探讨了其在 BCNI 大鼠模型中的修复潜力:结果:叔丁基对苯二酚(tBHQ)-ADSCs 在 BCNI 模型中的存活时间明显延长。此外,与其他组相比,叔丁基氢醌-ADSCs 组盆腔主要神经节(MPG)神经内容物和神经纤维的恢复更好,勃起功能改善更好,阴茎纤维化更少。此外,Nrf2和超氧化物歧化酶1(SOD1)的表达也高于其他组别:结论:Nrf2能增强ADSCs的抗氧化应激能力,从而提高ADSCs对BCNI大鼠模型的治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nrf2 enhances the therapeutic efficiency of adipose-derived stem cells in the treatment of neurogenic erectile dysfunction in a rat model.

Background: Erectile dysfunction (ED) caused by intraoperative nerve injury is a major complication of pelvic surgery. Adipose-derived stem cells (ADSCs) have presented therapeutic potential in a rat model of bilateral cavernous nerve injury (BCNI), while inadequate in vivo viability has largely limited their application. Nuclear factor-E2-related Factor (Nrf2) is a key transcription factor that regulates cellular anti-oxidative stress. In this work, we investigated the effect of Nrf2 expression regulation on the viability of ADSCs, and explore its repair potential in a BCNI rat model.

Results: The survival time of tert-Butylhydroquinone (tBHQ)-ADSCs in BCNI model increased obviously. In addition, the tBHQ-ADSCs group presented better restoration of major pelvic ganglion (MPG) nerve contents and fibers, better improvement of erectile function, and less penile fibrosis than the other groups. Moreover, the expression of Nrf2 and superoxide dismutase 1 (SOD1) were higher than those of other groups.

Conclusion: Nrf2 could enhance the anti-oxidative stress ability of ADSCs, so as to improve the therapeutic effect of ADSCs on BCNI rat model.

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来源期刊
Basic and Clinical Andrology
Basic and Clinical Andrology Medicine-Urology
CiteScore
3.50
自引率
0.00%
发文量
21
审稿时长
22 weeks
期刊介绍: Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.
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