利用护理点混合重组抗原免疫层析技术提高人类强直性脊柱炎的诊断灵敏度。

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2023-01-01 Epub Date: 2023-12-13 DOI:10.1051/parasite/2023063
Patcharaporn Boonroumkaew, Lakkhana Sadaow, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Punyisa Buadee, Chanida Suprom, Tongjit Thanchomnang, Pewpan M Intapan, Wanchai Maleewong
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引用次数: 0

摘要

斯特龙线虫病是一种被忽视的热带疾病,在免疫力低下的患者中可因过度感染和传播斯特龙线虫病而引起致命的并发症。我们利用两种盘尾丝虫重组抗原蛋白--L3NieAg.01(NIE)和 IgG-免疫反应抗原(SsIR),开发了基于重组抗原的免疫层析检测试剂盒(ICT)。我们构建并比较了使用 NIE(NIE ICT 试剂盒)或 SsIR(SsIR ICT 试剂盒)抗原的试剂盒,以及使用这两种抗原的混合试剂盒(NIE-SsIR ICT 试剂盒),以检测人血清样本中的抗斯通淋病 IgG 抗体。对正常健康人(I 组,n = 40)、已确诊的强直性脊柱炎患者(II 组,n = 100)和其他寄生虫感染者(III 组,n = 154)的血清样本进行了评估。NIE ICT试剂盒的灵敏度和特异性分别为81.0%和84.0%,SsIR ICT试剂盒的灵敏度和特异性分别为89.0%和83.5%,NIE-SsIR ICT试剂盒的灵敏度和特异性分别为95.0%和90.2%。NIE-SsIR ICT试剂盒的诊断结果最好;它可以作为粪便检查的补充,用于临床诊断,并可用于筛查流行地区高危人群中无症状的蠕形虫感染。NIE-SsIR ICT试剂盒还可用于流行地区的大规模血清流行病学调查,而无需额外的设施或辅助用品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based immunochromatography.

Strongyloidiasis is a neglected tropical disease that can cause fatal complications due to hyperinfection and disseminated strongyloidiasis in immunocompromised patients. We used two Strongyloides stercoralis recombinant antigenic proteins, L3NieAg.01 (NIE) and IgG-immunoreactive antigen (SsIR), to develop the recombinant antigen-based immunochromatography test (ICT) kit. We constructed and compared kits using either the NIE (NIE ICT kit) or the SsIR (SsIR ICT kit) antigens and a kit using a mixture of both (NIE-SsIR ICT kit) for detection of anti-Strongyloides IgG antibody in human serum samples. Serum samples from normal healthy individuals (Group I, n = 40), proven strongyloidiasis patients (Group II, n = 100), and those with other parasitic infections (Group III, n = 154) were evaluated. Sensitivity and specificity were 81.0% and 84.0% for the NIE ICT kit, 89.0% and 83.5% for the SsIR ICT kit, and 95.0% and 90.2% for the NIE-SsIR ICT kit, respectively. The NIE-SsIR ICT kit provided the best diagnostic results; it can supplement stool examination for clinical diagnosis and can be used to screen for asymptomatic S. stercoralis infection in people at risk in endemic areas. The NIE-SsIR ICT kit can also be used in large-scale sero-epidemiological investigations in endemic areas without the need for additional facilities or ancillary supplies.

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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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