Xuxin Chen , Fan Wang , Jian Tang , Jiguang Meng, Zhihai Han
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引用次数: 0
摘要
背景急性肺损伤（ALI）包括严重的肺损伤和呼吸衰竭，并伴随着肺泡巨噬细胞（AM）的活化。方法通过连续吸入脂多糖（LPS）建立 ALI 大鼠模型。分析了 PALM3 对大鼠存活率、肺损伤严重程度和巨噬细胞极化的影响。结果PALM3过表达会增加ALI大鼠的死亡率，加重肺部病理损伤，并促进AM向M1细胞极化。相反，PALM3 基因敲除则会产生相反的效果。结论在LPS诱导的ALI中，PALM3通过激活Notch信号通路加重肺损伤并诱导巨噬细胞向M1细胞极化，这可能对ALI/ARDS的治疗有所启示。
Paralemmin-3 augments lipopolysaccharide-induced acute lung injury with M1 macrophage polarization via the notch signaling pathway
Background
Acute lung injury (ALI) involves severe lung damage and respiratory failure, which are accompanied by alveolar macrophage (AM) activation. The aim of this article is to verify the influence of paralemmin-3 (PALM3) on alveolar macrophage (AM) polarization in ALI and the underlying mechanism of action.
Methods
An ALI rat model was established by successive lipopolysaccharide (LPS) inhalations. The influence of PALM3 on the survival rate, severity of lung injury, and macrophage polarization was analyzed. Furthermore, we explored the underlying mechanism of PALM3 in regulating macrophage polarization.
Results
PALM3 overexpression increased mortality of ALI rats, augmented lung pathological damage, and promoted AM polarization toward M1 cells. Conversely, PALM3 knockdown had the opposite effects. Mechanistically, PALM3 might promote M1 polarization by acting as an adaptor to facilitate transduction of Notch signaling.
Conclusion
PALM3 aggravates lung injury and induces macrophage polarization toward M1 cells by activating the Notch signaling pathway in LPS-induced ALI, which may shed light on ALI/ARDS treatments.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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