基于细胞挤压和电场协同作用的微流体细胞内输送模拟

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Jianfeng Chen, Han Liu, Chuan Li, Xiaoxiao Chen, Yichuan Dai
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引用次数: 0

摘要

近年来,用于细胞内递送的微流体挤压法已显示出高效性和通用性。然而,这种方法在有效转染大分子方面仍面临困难。将这种方法与其他膜破坏策略相结合,可以提高细胞内递送效率和细胞活力。值得注意的是,微通道挤压和电场的结合是最关键的策略。细胞膜在微流体装置中迅速穿孔,然后引入电场,进一步提高质膜的通透性,使不通透的分子得以跨膜转运。然而,挤压和电穿孔相结合的方法对细胞膜失稳和物质转运的潜在机制仍不清楚。因此,本文旨在建立一个计算模型,研究各种外部刺激对细胞内输送过程的影响,并探讨混合外部刺激的影响以及电穿孔和挤压对细胞内输送的不同影响。同时,我们修改了挤压参数(微通道尺寸和细胞速度)和电场参数(脉冲长度、电场强度等),以优化细胞对外来物质的吸收。模拟结果表明,收缩宽度的减小、收缩长度的增加和细胞平均速度的提高可促进细胞的挤压变形以及细胞膜上孔隙的形成。细胞挤压和电场的共同作用增强了细胞对物质的吸收。此外,电参数的变化也会影响细胞挤压与电场共同作用的结果。例如,增加电场脉冲的长度可提高细胞膜的通透性。不过,电场强度必须设定在合理的范围内(几千伏/厘米),以防止细胞失活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Simulation of microfluidic intracellular delivery based on the synergy of cell squeezing and electrical field

Simulation of microfluidic intracellular delivery based on the synergy of cell squeezing and electrical field

In recent years, the microfluidic squeezing method for cell intracellular delivery has demonstrated high efficiency and generalizability. This approach, however, still faces difficulties in effectively transfecting large molecules. Integration of this method with other membrane disruption strategies can enhance intracellular delivery efficiency and cell viability. Notably, the combination of microchannel squeezing and electric fields emerges as the most crucial strategy. The cell membrane is rapidly perforated in a microfluidic device, and then an electric field is introduced to further improve the permeability of the plasma membrane, allowing transmembrane transit of impermeable molecules. Nevertheless, the underlying mechanism of the combined squeezing and electroporation method on cell membrane destabilization and material transport remains unclear. Thus, this paper aims to develop a computational model to investigate the intracellular delivery process influenced by various external stimuli and to examine the implications of mixing external stimuli as well as the distinct effects of electric and squeezing on intracellular delivery. Meanwhile, we modified the squeezing parameters (microchannel size and cell velocity) and the electric field parameters (pulse length, electric field strength, etc.) to optimize the cell’s absorption of foreign substances. The simulation results indicate that a decrease in the contraction width, an increase in the contraction length, and an increase in the average cell velocity could promote the squeezing deformation of the cell as well as the formation of pores on the cell membrane. And the joint action of cell squeezing and electric field enhances cellular absorption of substances. In addition, the change of electrical parameters also affects the results of cell squeezing in conjunction with the electric field. For example, the increased length of electric field pulses improves the cell membrane permeability. However, the electric field intensity must be set in a reasonable range (< several kV/cm) to prevent cell inactivation.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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