Yifan Fei , Wenyi Yang , Zhaoyang Guo , Haishui Sun , Fan Yang , Jingzhou Hu
{"title":"凹槽 \"结构微弧氧化钛的细胞粘附、成骨和血管生成研究","authors":"Yifan Fei , Wenyi Yang , Zhaoyang Guo , Haishui Sun , Fan Yang , Jingzhou Hu","doi":"10.1016/j.apsadv.2023.100552","DOIUrl":null,"url":null,"abstract":"<div><p>The use of titanium alloy-based dental implant restorations has gained popularity due to their attractive properties. Current research on the surface modification of titanium materials primarily centers around the surface integration of various metal ions, the incorporation of different drugs, or other materials. By simply adjusting the process parameters of micro-arc oxidation, we were able to form a “groove” structure in titanium chips. Scanning Electron Microscopy (SEM) observations revealed that Bone Marrow Stem Cells (BMSCs) noticeably elongated along the “grooves”. Immunofluorescence results indicated an elevated expression of Osteocalcin (OCN) and CD31 in “groove” structure group. Furthermore, “groove” structure group also amplified the expression of osteogenic genes (Alkaline Phosphatase, ALP; Osteocalcin, OCN) and angiogenic genes (CD31, Vascular Endothelial Growth Factor, VEGF; Angiopoietin-2, ANG2; and Fibroblast Growth Factor, FGF) on the material surface (<em>P</em> < 0.05). This study suggests that the “groove” structure enhances early cell adhesion on the material surface and improves osteogenic and angiogenic differentiation on the titanium surface, thereby providing potential research implications for enhancing the initial stability of implants.</p></div>","PeriodicalId":34303,"journal":{"name":"Applied Surface Science Advances","volume":"19 ","pages":"Article 100552"},"PeriodicalIF":7.5000,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666523923001861/pdfft?md5=fe41d9207b7a640e20d9e9261a93fd6b&pid=1-s2.0-S2666523923001861-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Study of cell adhesion, osteogenesis, and angiogenesis of a “groove” structure micro-arc oxidation titanium\",\"authors\":\"Yifan Fei , Wenyi Yang , Zhaoyang Guo , Haishui Sun , Fan Yang , Jingzhou Hu\",\"doi\":\"10.1016/j.apsadv.2023.100552\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The use of titanium alloy-based dental implant restorations has gained popularity due to their attractive properties. Current research on the surface modification of titanium materials primarily centers around the surface integration of various metal ions, the incorporation of different drugs, or other materials. By simply adjusting the process parameters of micro-arc oxidation, we were able to form a “groove” structure in titanium chips. Scanning Electron Microscopy (SEM) observations revealed that Bone Marrow Stem Cells (BMSCs) noticeably elongated along the “grooves”. Immunofluorescence results indicated an elevated expression of Osteocalcin (OCN) and CD31 in “groove” structure group. Furthermore, “groove” structure group also amplified the expression of osteogenic genes (Alkaline Phosphatase, ALP; Osteocalcin, OCN) and angiogenic genes (CD31, Vascular Endothelial Growth Factor, VEGF; Angiopoietin-2, ANG2; and Fibroblast Growth Factor, FGF) on the material surface (<em>P</em> < 0.05). This study suggests that the “groove” structure enhances early cell adhesion on the material surface and improves osteogenic and angiogenic differentiation on the titanium surface, thereby providing potential research implications for enhancing the initial stability of implants.</p></div>\",\"PeriodicalId\":34303,\"journal\":{\"name\":\"Applied Surface Science Advances\",\"volume\":\"19 \",\"pages\":\"Article 100552\"},\"PeriodicalIF\":7.5000,\"publicationDate\":\"2023-12-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666523923001861/pdfft?md5=fe41d9207b7a640e20d9e9261a93fd6b&pid=1-s2.0-S2666523923001861-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Surface Science Advances\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666523923001861\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Surface Science Advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666523923001861","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Study of cell adhesion, osteogenesis, and angiogenesis of a “groove” structure micro-arc oxidation titanium
The use of titanium alloy-based dental implant restorations has gained popularity due to their attractive properties. Current research on the surface modification of titanium materials primarily centers around the surface integration of various metal ions, the incorporation of different drugs, or other materials. By simply adjusting the process parameters of micro-arc oxidation, we were able to form a “groove” structure in titanium chips. Scanning Electron Microscopy (SEM) observations revealed that Bone Marrow Stem Cells (BMSCs) noticeably elongated along the “grooves”. Immunofluorescence results indicated an elevated expression of Osteocalcin (OCN) and CD31 in “groove” structure group. Furthermore, “groove” structure group also amplified the expression of osteogenic genes (Alkaline Phosphatase, ALP; Osteocalcin, OCN) and angiogenic genes (CD31, Vascular Endothelial Growth Factor, VEGF; Angiopoietin-2, ANG2; and Fibroblast Growth Factor, FGF) on the material surface (P < 0.05). This study suggests that the “groove” structure enhances early cell adhesion on the material surface and improves osteogenic and angiogenic differentiation on the titanium surface, thereby providing potential research implications for enhancing the initial stability of implants.