Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi
{"title":"RpoN(sigma factor 54)有助于支气管败血波氏杆菌气管定植过程中的细菌适应性","authors":"Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi","doi":"10.1111/1348-0421.13109","DOIUrl":null,"url":null,"abstract":"<p>The Gram-negative pathogenic bacterium <i>Bordetella bronchiseptica</i> is a respiratory pathogen closely related to <i>Bordetella pertussis</i>, the causative agent of whooping cough. Despite sharing homologous virulence factors, <i>B. bronchiseptica</i> infects a broad range of mammalian hosts, including some experimental animals, whereas <i>B. pertussis</i> is strictly adapted to humans. Therefore, <i>B. bronchiseptica</i> is often used as a representative model to explore the pathogenicity of <i>Bordetella</i> in infection experiments with laboratory animals. Although <i>Bordetella</i> virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a <i>rpoN</i>-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. <i>rpoN</i> encodes σ<sup>54</sup>, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of <i>B. bronchiseptica</i> is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13109","citationCount":"0","resultStr":"{\"title\":\"RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica\",\"authors\":\"Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi\",\"doi\":\"10.1111/1348-0421.13109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The Gram-negative pathogenic bacterium <i>Bordetella bronchiseptica</i> is a respiratory pathogen closely related to <i>Bordetella pertussis</i>, the causative agent of whooping cough. Despite sharing homologous virulence factors, <i>B. bronchiseptica</i> infects a broad range of mammalian hosts, including some experimental animals, whereas <i>B. pertussis</i> is strictly adapted to humans. Therefore, <i>B. bronchiseptica</i> is often used as a representative model to explore the pathogenicity of <i>Bordetella</i> in infection experiments with laboratory animals. Although <i>Bordetella</i> virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a <i>rpoN</i>-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. <i>rpoN</i> encodes σ<sup>54</sup>, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of <i>B. bronchiseptica</i> is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.</p>\",\"PeriodicalId\":18679,\"journal\":{\"name\":\"Microbiology and Immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13109\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology and Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13109\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology and Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13109","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica
The Gram-negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. rpoN encodes σ54, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.
期刊介绍:
Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses.
Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.