RpoN(sigma factor 54)有助于支气管败血波氏杆菌气管定植过程中的细菌适应性

IF 1.9 4区 医学 Q4 IMMUNOLOGY
Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi
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引用次数: 0

摘要

革兰氏阴性致病细菌支气管败血波氏杆菌是一种呼吸道病原体,与百日咳的病原体百日咳波氏杆菌密切相关。尽管具有相同的致病因子,支气管败血波氏杆菌可感染多种哺乳动物宿主,包括一些实验动物,而百日咳波氏杆菌则严格适应于人类。因此,支气管败血波氏杆菌经常被用作实验动物感染实验中探索博德特氏菌致病性的代表性模型。尽管包括毒素和粘附素在内的博德特氏菌毒力因子已被深入研究,但我们最近的研究表明,未知的毒力因子参与了气管定植和感染。在此,我们通过高通量转座子测序(Tn-seq)研究了导致气管定植的细菌基因。经过筛选,我们发现了151个具有不同功能的候选基因,并发现rpoN缺失突变株与野生型菌株共同接种时,气管定殖功能存在缺陷。事实上,我们发现支气管败血波氏杆菌的 RpoN 参与了细菌的运动和初始生物膜的形成。根据这些结果,我们认为 RpoN 通过调节各种细菌功能来支持细菌的定植。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica

The Gram-negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. rpoN encodes σ54, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.

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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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