{"title":"渗透胁迫、光照光谱和温度对离体野生草莓(Fragaria vesca L.)生长及花青素生物合成途径相关基因表达的影响","authors":"Jurgita Vinskienė, Vidmantas Bendokas, Vidmantas Stanys, Audrius Sasnauskas, Rytis Rugienius","doi":"10.2478/fhort-2023-0030","DOIUrl":null,"url":null,"abstract":"The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of <jats:italic>Fragaria vesca in vitro</jats:italic> and the expression of regulatory <jats:italic>Myb10, WD40</jats:italic> and enzyme-coding genes <jats:italic>CHI, CHS, DFR, EGL, F3H</jats:italic> and <jats:italic>UFGT</jats:italic>, which are essential for anthocyanin biosynthesis. We observed plants’ response to osmotic stress, the decrease in growth and microshoot weight. A change in the expression of the investigated genes was evident under the suboptimal concentration of sucrose. The addition of PEG to the medium caused a decrease in microshoot weight and gene expression. Blue + red lights of the LED lighting system significantly affected microshoot growth <jats:italic>in vitro</jats:italic>. Red and blue + red + UV lights slightly reduced microshoot weight and caused a reddish colour of petioles, which indicate increased anthocyanin synthesis. Moreover, most of the studied genes’ expression tended to increase when shoots were exposed to blue, blue + red and blue + red + UV lights. A temperature of 15°C (vs 22°C) significantly reduced the mean fresh weight of microshoots while increasing <jats:italic>CHI</jats:italic> and <jats:italic>CHS</jats:italic> gene expression and decreasing <jats:italic>WD40</jats:italic> gene expression. Exposure to a higher temperature (30°C) induced the vitrification of microshoots, although the fresh weight did not differ from that of the control. Gene expression also depended on the duration of exposure. In the case of <jats:italic>CHS</jats:italic>, gene expression remained the same or increased after exposure for 1 week and then decreased after exposure for 4 weeks.","PeriodicalId":12277,"journal":{"name":"Folia Horticulturae","volume":"71 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro\",\"authors\":\"Jurgita Vinskienė, Vidmantas Bendokas, Vidmantas Stanys, Audrius Sasnauskas, Rytis Rugienius\",\"doi\":\"10.2478/fhort-2023-0030\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of <jats:italic>Fragaria vesca in vitro</jats:italic> and the expression of regulatory <jats:italic>Myb10, WD40</jats:italic> and enzyme-coding genes <jats:italic>CHI, CHS, DFR, EGL, F3H</jats:italic> and <jats:italic>UFGT</jats:italic>, which are essential for anthocyanin biosynthesis. We observed plants’ response to osmotic stress, the decrease in growth and microshoot weight. A change in the expression of the investigated genes was evident under the suboptimal concentration of sucrose. The addition of PEG to the medium caused a decrease in microshoot weight and gene expression. Blue + red lights of the LED lighting system significantly affected microshoot growth <jats:italic>in vitro</jats:italic>. Red and blue + red + UV lights slightly reduced microshoot weight and caused a reddish colour of petioles, which indicate increased anthocyanin synthesis. Moreover, most of the studied genes’ expression tended to increase when shoots were exposed to blue, blue + red and blue + red + UV lights. A temperature of 15°C (vs 22°C) significantly reduced the mean fresh weight of microshoots while increasing <jats:italic>CHI</jats:italic> and <jats:italic>CHS</jats:italic> gene expression and decreasing <jats:italic>WD40</jats:italic> gene expression. Exposure to a higher temperature (30°C) induced the vitrification of microshoots, although the fresh weight did not differ from that of the control. Gene expression also depended on the duration of exposure. In the case of <jats:italic>CHS</jats:italic>, gene expression remained the same or increased after exposure for 1 week and then decreased after exposure for 4 weeks.\",\"PeriodicalId\":12277,\"journal\":{\"name\":\"Folia Horticulturae\",\"volume\":\"71 1\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-12-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/fhort-2023-0030\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/fhort-2023-0030","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro
The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of Fragaria vesca in vitro and the expression of regulatory Myb10, WD40 and enzyme-coding genes CHI, CHS, DFR, EGL, F3H and UFGT, which are essential for anthocyanin biosynthesis. We observed plants’ response to osmotic stress, the decrease in growth and microshoot weight. A change in the expression of the investigated genes was evident under the suboptimal concentration of sucrose. The addition of PEG to the medium caused a decrease in microshoot weight and gene expression. Blue + red lights of the LED lighting system significantly affected microshoot growth in vitro. Red and blue + red + UV lights slightly reduced microshoot weight and caused a reddish colour of petioles, which indicate increased anthocyanin synthesis. Moreover, most of the studied genes’ expression tended to increase when shoots were exposed to blue, blue + red and blue + red + UV lights. A temperature of 15°C (vs 22°C) significantly reduced the mean fresh weight of microshoots while increasing CHI and CHS gene expression and decreasing WD40 gene expression. Exposure to a higher temperature (30°C) induced the vitrification of microshoots, although the fresh weight did not differ from that of the control. Gene expression also depended on the duration of exposure. In the case of CHS, gene expression remained the same or increased after exposure for 1 week and then decreased after exposure for 4 weeks.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.