对 COVID-19 感染者和健康对照组男性精子甲基化模式变化的评估

Isaac Stirland B.S. , Murilo Racy Soares Ph.D. , Cristiana Libardi Miranda Furtado Ph.D. , Rosana Maria Dos Reis Ph.D. , Kenneth I. Aston Ph.D. , R. Parker Smith , Timothy G. Jenkins Ph.D.
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引用次数: 0

摘要

目的 考虑到导致半全球范围内男性生育能力下降的许多潜在因素,确定严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)感染是否会影响男性生殖健康。设计共对 64 份人类精液样本(32 份治疗样本和 32 份对照样本)进行了实验室处理和生物信息学分析,以评估 DNA 甲基化模式的差异。利用多种生物信息学工具进行分析,除了甲基化模式与感染后时间的关系外,还将得出组间在表观遗传年龄、表观遗传不稳定性、半全局和区域甲基化方面的差异。患者64人的研究队列是从圣保罗大学里贝拉普雷图医学院临床医院人类生殖中心于2021年6月至2022年1月期间招募的94名志愿者中抽取出来的。对人类精母细胞关键调控位点的平均β值进行分析,并进行组间比较。使用表观遗传学年龄、不稳定性、半全局性和区域甲基化工具对β值进行进一步分析,提供了具有广度和深度的分析结果。考虑到这些结果,可以推断感染 SARS-CoV-2 并不会显著和/或持续改变人类精母细胞的表观遗传组。从另一个角度看,这些数据也表明,病毒对人类男性生殖健康的改变微乎其微,或者说这种改变与表观遗传学程序无关。本研究认为,这种改变并不具有表观遗传学基础,而很可能是伴随症状(即发烧和炎症)的结果。在进行的多项生物信息学分析中,该检测结果未发现2019年冠状病毒病与非冠状病毒病精液供体组之间的DNA甲基化模式存在任何差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An assessment of alterations to human sperm methylation patterns in coronavirus disease 2019 infected and healthy control males

Objective

To determine whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection affects male reproductive health, considering the many potential factors that contribute to declines in male fertility on a semiglobal scale.

Design

In total, 64 human semen samples—32 treatment and 32 control—were laboratory processed and bioinformatically analyzed to assess differences in DNA methylation patterns. Implementing multiple bioinformatic tools, the analyses conducted will elicit between-group differences with respect to epigenetic age, epigenetic instability, semiglobal, and regional methylation, in addition to methylation patterns as a function of time since infection.

Setting

University hospital.

Patients

The study cohort of 64 individuals was drawn from a larger population of 94 volunteer participants recruited at the Human Reproduction Center at the Clinical Hospital of the Ribeirao Preto Medical School—University of São Paulo between June 2021 and January 2022 as well as in accordance with the ethical guidelines established by the Declaration of Helsinki.

Intervention

Exposure to SARS-CoV-2.

Main Outcome Measure(s)

Effects on male reproductive health were reported as differences in DNA methylation measured using an array. Mean β values at key regulatory loci for human spermatocytes were analyzed and compared between groups. Further analysis of β values using epigenetic age, instability, semiglobal, and regional methylation tools provided an analysis with substantial breadth and depth.

Results

In all analyses, there were no differences between groups. Considering these results, it can be inferred that infection with SARS-CoV-2 does not alter the epigenome of human spermatocytes in significant and/or persistent ways. Tangentially, these data also suggest that human male reproductive health is minimally altered by the virus, or that it is altered in a way that is independent of epigenetic programming.

Conclusion

Infection with SARS-CoV-2 has been reportedly associated with alterations in male fertility. This study asserts that such alterations do not have an epigenetic basis but are likely a result of concomitant symptomatology, i.e., fever and inflammation. Across the multiple bioinformatic analyses conducted, the results of this test did not detect any differences in DNA methylation patterns between coronavirus disease 2019 and noncoronavirus disease semen donor groups.

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来源期刊
F&S science
F&S science Endocrinology, Diabetes and Metabolism, Obstetrics, Gynecology and Women's Health, Urology
CiteScore
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审稿时长
51 days
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