评估红海海洋海绵 Xestospongia Testudinaria 的甲醇提取物及其相关化合物对 MCF-7 人类乳腺癌细胞的细胞毒性

IF 3.3 4区 医学 Q2 ONCOLOGY
Faris A Alkhilaiwi, Sana Fadil, Fadwa Aljoud, Ahmed Yonbawi, Abrar Ashi, Rawan H Hareeri, S. Bakhashab, Ahmad Alamri, Ahmed Albikairi, L. Shaala, Ali El-Gamal, Diaa Youssef
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引用次数: 0

摘要

乳腺癌是导致死亡的主要原因,也是世界上最常见的致命疾病之一。在红海海绵体中发现了多种化合物,包括甾醇酯、甾醇、吲哚生物碱和溴化多不饱和脂肪酸。这些化合物已被证明具有良好的生物学特性,包括抗炎、抗癌和抗氧化能力。方法采用3-(4,5-二甲基噻唑-2-酰基)-2,5-二苯基溴化四唑(MTT)测定和MCF-7细胞株的形态学变化,评价绒绵菌的细胞毒性。此外,流式细胞术还用于评估细胞凋亡和确定细胞周期的变化;此外,通过抓伤愈合实验评估细胞迁移。结果MCF-7细胞存活率呈剂量依赖性降低,IC50为39.8 ug/mL。在MCF-7上进行的功能研究表明,在刮痕实验中,绒绵虫在G1/G0时增加凋亡细胞死亡并诱导生长停滞,同时抑制细胞迁移。结论绒海绵提取物具有抑制乳腺癌细胞增殖、迁移和诱导凋亡的作用。因此,它作为一种潜在的乳腺癌治疗方法具有很大的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Cytotoxicity of the Methanolic Extract of Red Sea Marine Sponge Xestospongia Testudinaria and Its Related Compounds Against MCF-7 Human Breast Cancer Cells
Background Breast cancer is a leading cause of death and one of the most common fatal medical conditions in the world. Chemical compounds of various types have been identified in the Red Sea marine sponge Xestospongia testudinaria, including sterol esters, sterols, indole alkaloids, and brominated polyunsaturated fatty acids. These compounds have demonstrated promising biological features, which in cludes anti-inflammatory, cancer preventive, and antioxidant capacities. Methods The cytotoxic potential of Xestospongia testudinaria was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphological alterations in MCF-7 cell line. Furthermore, the flow cytometry was also utilized to assess apoptosis and identify changes in the cell cycle; besides, cell migration was assessed by scratch wound-healing assay. Results A significant dose-dependent decrease in the percentage of MCF-7 cell viability was observed with IC50 39.8 ug/mL. Functional studies were performed on MCF-7 to show that Xestospongia testudinaria raises apoptotic cell death and induces growth arrest at the G1/G0 while inhibiting cell migration in scratch assay. Conclusion These results demonstrated that Xestospongia testudinaria extract has an inhibitory effect on breast cancer cells proliferation, migration and induce apoptosis. Thus, it holds great promise as a potential treatment for breast cancer.
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CiteScore
4.10
自引率
0.00%
发文量
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审稿时长
16 weeks
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