不锈钢绒上的酶固定化及其对旋转填料床反应器中酶催化的传质增效作用

IF 3.8 3区 化学 Q2 CHEMISTRY, PHYSICAL
Catalysts Pub Date : 2023-12-08 DOI:10.3390/catal13121501
Ruiyi Yang, Juntao Xu, Jinglong Wu, Dong Lu, Fang Wang, Kaili Nie
{"title":"不锈钢绒上的酶固定化及其对旋转填料床反应器中酶催化的传质增效作用","authors":"Ruiyi Yang, Juntao Xu, Jinglong Wu, Dong Lu, Fang Wang, Kaili Nie","doi":"10.3390/catal13121501","DOIUrl":null,"url":null,"abstract":"Rotating packed beds (RPB) facilitate the mixing of heterogeneous substrates, and promote high mass transfer efficiency in heterogeneous reactions. For the enzymatic reactions, traditional porous particles with immobilized enzymes are sensitive to the strong sheer force of the RPB, thus limiting its application. This work offers a strategy for enzyme immobilization on the surface of stainless-steel fleece, to improve the shear strength resistance of immobilized enzymes. Lipase was applied to investigate and optimize the immobilization. Finally, a fatty acid hydratase (FAH) was applied for immobilization based on the optimized method, which was further applied for evaluating its performance in RPB. The results indicated that metal immobilized enzymes resist a higher shear force than their particle-immobilized alternatives. Operating at a centrifugal force factor (β) of 30, the hydration conversion rate of 96% is achieved after 8 h, which was from nearly 38% faster than in a stirrer tank reactor (hydration yield of 60%). The metal immobilization, moreover, efficiently improved the enzyme reusability, as demonstrated by a conversion rate remaining above 90% after 15 batches. These results indicated that a metal immobilization method combined with an RPB reactor significantly increases the efficiency of enzymatic reactions.","PeriodicalId":9794,"journal":{"name":"Catalysts","volume":"32 1","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enzyme Immobilization on Stainless Steel Fleece and Its Mass Transfer Enhancement of Enzymatic Catalysis in a Rotating Packed Bed Reactor\",\"authors\":\"Ruiyi Yang, Juntao Xu, Jinglong Wu, Dong Lu, Fang Wang, Kaili Nie\",\"doi\":\"10.3390/catal13121501\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rotating packed beds (RPB) facilitate the mixing of heterogeneous substrates, and promote high mass transfer efficiency in heterogeneous reactions. For the enzymatic reactions, traditional porous particles with immobilized enzymes are sensitive to the strong sheer force of the RPB, thus limiting its application. This work offers a strategy for enzyme immobilization on the surface of stainless-steel fleece, to improve the shear strength resistance of immobilized enzymes. Lipase was applied to investigate and optimize the immobilization. Finally, a fatty acid hydratase (FAH) was applied for immobilization based on the optimized method, which was further applied for evaluating its performance in RPB. The results indicated that metal immobilized enzymes resist a higher shear force than their particle-immobilized alternatives. Operating at a centrifugal force factor (β) of 30, the hydration conversion rate of 96% is achieved after 8 h, which was from nearly 38% faster than in a stirrer tank reactor (hydration yield of 60%). The metal immobilization, moreover, efficiently improved the enzyme reusability, as demonstrated by a conversion rate remaining above 90% after 15 batches. These results indicated that a metal immobilization method combined with an RPB reactor significantly increases the efficiency of enzymatic reactions.\",\"PeriodicalId\":9794,\"journal\":{\"name\":\"Catalysts\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2023-12-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Catalysts\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3390/catal13121501\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Catalysts","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/catal13121501","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

摘要

旋转填料床(RPB)促进了非均相底物的混合,提高了非均相反应的传质效率。对于酶促反应,传统的固定化酶的多孔颗粒对RPB的强绝对力敏感,限制了其应用。本研究为不锈钢起绒表面的酶固定化提供了一种策略,以提高固定化酶的抗剪切强度。应用脂肪酶对固定化酶进行研究和优化。最后,将优化后的方法应用于脂肪酸水合酶(FAH)的固定化,并对其在RPB中的性能进行了评价。结果表明,金属固定化酶比颗粒固定化酶承受更高的剪切力。在离心力因子(β)为30的条件下,8 h的水化转化率为96%,比搅拌槽反应器(水化率为60%)提高了近38%。此外,金属固定有效地提高了酶的可重复使用性,在15批次后转化率保持在90%以上。这些结果表明,金属固定化方法与RPB反应器相结合可以显著提高酶促反应的效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enzyme Immobilization on Stainless Steel Fleece and Its Mass Transfer Enhancement of Enzymatic Catalysis in a Rotating Packed Bed Reactor
Rotating packed beds (RPB) facilitate the mixing of heterogeneous substrates, and promote high mass transfer efficiency in heterogeneous reactions. For the enzymatic reactions, traditional porous particles with immobilized enzymes are sensitive to the strong sheer force of the RPB, thus limiting its application. This work offers a strategy for enzyme immobilization on the surface of stainless-steel fleece, to improve the shear strength resistance of immobilized enzymes. Lipase was applied to investigate and optimize the immobilization. Finally, a fatty acid hydratase (FAH) was applied for immobilization based on the optimized method, which was further applied for evaluating its performance in RPB. The results indicated that metal immobilized enzymes resist a higher shear force than their particle-immobilized alternatives. Operating at a centrifugal force factor (β) of 30, the hydration conversion rate of 96% is achieved after 8 h, which was from nearly 38% faster than in a stirrer tank reactor (hydration yield of 60%). The metal immobilization, moreover, efficiently improved the enzyme reusability, as demonstrated by a conversion rate remaining above 90% after 15 batches. These results indicated that a metal immobilization method combined with an RPB reactor significantly increases the efficiency of enzymatic reactions.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Catalysts
Catalysts CHEMISTRY, PHYSICAL-
CiteScore
6.80
自引率
7.70%
发文量
1330
审稿时长
3 months
期刊介绍: Catalysts (ISSN 2073-4344) is an international open access journal of catalysts and catalyzed reactions. Catalysts publishes reviews, regular research papers (articles) and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信