揭示海洋中钴胺素的光降解过程

IF 5.1 2区 地球科学 Q1 LIMNOLOGY
Catherine C. Bannon, Elizabeth M. Mudge, Erin M. Bertrand
{"title":"揭示海洋中钴胺素的光降解过程","authors":"Catherine C. Bannon,&nbsp;Elizabeth M. Mudge,&nbsp;Erin M. Bertrand","doi":"10.1002/lol2.10371","DOIUrl":null,"url":null,"abstract":"<p>Cobalamin, vitamin B<sub>12</sub>, is an important micronutrient that has been investigated for decades in the marine context because it is required for phytoplankton growth. The biologically active forms (Me-B<sub>12</sub>, Ado-B<sub>12</sub>) and the synthetic form (CN-B<sub>12</sub>) quickly convert to OH-B<sub>12</sub> after light exposure in various aqueous solutions, but puzzlingly have been frequently reported to dominate dissolved cobalamin pools in the sunlit ocean. Here, we document photodegradation timescales for these cobalamin forms in natural seawater using targeted mass spectrometry, providing quantitative evidence that OH-B<sub>12</sub> is expected to be the dominant dissolved form in irradiated seawater. Then, through high-resolution mass spectrometry, we identify four photodegradation products of OH-B<sub>12</sub> which represent potential building blocks microbes could salvage and remodel to satisfy cellular cobalamin requirements. Taken together, these results clarify the impact of light on marine cobalamin dynamics, laying a foundation for a more quantitative understanding of the role of cobalamin in microbial communities and biogeochemical cycles.</p>","PeriodicalId":18128,"journal":{"name":"Limnology and Oceanography Letters","volume":null,"pages":null},"PeriodicalIF":5.1000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lol2.10371","citationCount":"0","resultStr":"{\"title\":\"Shedding light on cobalamin photodegradation in the ocean\",\"authors\":\"Catherine C. Bannon,&nbsp;Elizabeth M. Mudge,&nbsp;Erin M. Bertrand\",\"doi\":\"10.1002/lol2.10371\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Cobalamin, vitamin B<sub>12</sub>, is an important micronutrient that has been investigated for decades in the marine context because it is required for phytoplankton growth. The biologically active forms (Me-B<sub>12</sub>, Ado-B<sub>12</sub>) and the synthetic form (CN-B<sub>12</sub>) quickly convert to OH-B<sub>12</sub> after light exposure in various aqueous solutions, but puzzlingly have been frequently reported to dominate dissolved cobalamin pools in the sunlit ocean. Here, we document photodegradation timescales for these cobalamin forms in natural seawater using targeted mass spectrometry, providing quantitative evidence that OH-B<sub>12</sub> is expected to be the dominant dissolved form in irradiated seawater. Then, through high-resolution mass spectrometry, we identify four photodegradation products of OH-B<sub>12</sub> which represent potential building blocks microbes could salvage and remodel to satisfy cellular cobalamin requirements. Taken together, these results clarify the impact of light on marine cobalamin dynamics, laying a foundation for a more quantitative understanding of the role of cobalamin in microbial communities and biogeochemical cycles.</p>\",\"PeriodicalId\":18128,\"journal\":{\"name\":\"Limnology and Oceanography Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2023-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lol2.10371\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Limnology and Oceanography Letters\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/lol2.10371\",\"RegionNum\":2,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"LIMNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography Letters","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lol2.10371","RegionNum":2,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"LIMNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

钴胺素,维生素B12,是一种重要的微量营养素,已经在海洋环境中研究了几十年,因为它是浮游植物生长所必需的。生物活性形式(Me-B12, Ado-B12)和合成形式(CN-B12)在各种水溶液中光照后迅速转化为OH-B12,但令人困惑的是,经常有报道称在阳光照射的海洋中溶解的钴胺素池占主导地位。在这里,我们使用靶向质谱法记录了天然海水中这些钴胺素形式的光降解时间尺度,提供了定量证据,表明OH-B12有望成为辐照海水中主要的溶解形式。然后,通过高分辨率质谱分析,我们鉴定了OH-B12的四种光降解产物,它们代表了微生物可以修复和改造以满足细胞钴胺素需求的潜在构建块。综上所述,这些结果阐明了光对海洋钴胺素动力学的影响,为更定量地了解钴胺素在微生物群落和生物地球化学循环中的作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Shedding light on cobalamin photodegradation in the ocean

Shedding light on cobalamin photodegradation in the ocean

Cobalamin, vitamin B12, is an important micronutrient that has been investigated for decades in the marine context because it is required for phytoplankton growth. The biologically active forms (Me-B12, Ado-B12) and the synthetic form (CN-B12) quickly convert to OH-B12 after light exposure in various aqueous solutions, but puzzlingly have been frequently reported to dominate dissolved cobalamin pools in the sunlit ocean. Here, we document photodegradation timescales for these cobalamin forms in natural seawater using targeted mass spectrometry, providing quantitative evidence that OH-B12 is expected to be the dominant dissolved form in irradiated seawater. Then, through high-resolution mass spectrometry, we identify four photodegradation products of OH-B12 which represent potential building blocks microbes could salvage and remodel to satisfy cellular cobalamin requirements. Taken together, these results clarify the impact of light on marine cobalamin dynamics, laying a foundation for a more quantitative understanding of the role of cobalamin in microbial communities and biogeochemical cycles.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
10.00
自引率
3.80%
发文量
63
审稿时长
25 weeks
期刊介绍: Limnology and Oceanography Letters (LO-Letters) serves as a platform for communicating the latest innovative and trend-setting research in the aquatic sciences. Manuscripts submitted to LO-Letters are expected to present high-impact, cutting-edge results, discoveries, or conceptual developments across all areas of limnology and oceanography, including their integration. Selection criteria for manuscripts include their broad relevance to the field, strong empirical and conceptual foundations, succinct and elegant conclusions, and potential to advance knowledge in aquatic sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信