口服双歧杆菌可通过上调白细胞介素-10改善抗血管生成药物引起的口腔黏膜伤口愈合障碍

IF 10.8 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Qingxiang Li, Yuke Li, Qiao Qiao, Ning Zhao, Yuanning Yang, Lin Wang, Yifei Wang, Chuanbin Guo, Yuxing Guo
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引用次数: 0

摘要

最近的研究表明,长期应用抗血管生成药物可能会损害口腔黏膜伤口愈合。本研究探讨了舒尼替尼对小鼠口腔黏膜愈合障碍的影响以及前列双歧杆菌(B. breve)的治疗潜力。小鼠硬腭粘膜缺损模型用于研究舒尼替尼和/或唑来膦酸钠对伤口愈合的影响。通过微型计算机断层扫描(micro-CT)评估了粘膜缺损下骨的体积和密度。通过蛋白质芯片分析和酶联免疫吸附试验(ELISA)检测炎症因子。用舒尼替尼处理的口腔黏膜干细胞(OMSCs)的衰老和生物功能进行了测试。接合环实验用于研究口服布氏杆菌的影响。使用白细胞介素-10(IL-10)的中和抗体证明了IL-10在前列茵促愈合过程中的关键作用。结果显示,舒尼替尼导致小鼠口腔黏膜伤口愈合受损。在体外,舒尼替尼诱导 OMSCs 细胞衰老,并影响增殖、迁移和分化等生物功能。通过肠道树突状细胞(DCs)产生的IL-10,口服布瑞韦能减轻口腔黏膜炎症并促进伤口愈合。IL-10能逆转舒尼替尼导致的OMSCs细胞衰老,IL-10中和抗体能阻断布氏酵母菌在舒尼替尼治疗条件下对口腔黏膜伤口愈合的改善作用。总之,舒尼替尼可诱导OMSCs细胞衰老并导致口腔黏膜伤口愈合受损,而口服布氏杆菌可通过肠道DCs衍生的IL-10改善伤口愈合受损。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oral administration of Bifidobacterium breve improves anti-angiogenic drugs-derived oral mucosal wound healing impairment via upregulation of interleukin-10

Oral administration of Bifidobacterium breve improves anti-angiogenic drugs-derived oral mucosal wound healing impairment via upregulation of interleukin-10

Recent studies have suggested that long-term application of anti-angiogenic drugs may impair oral mucosal wound healing. This study investigated the effect of sunitinib on oral mucosal healing impairment in mice and the therapeutic potential of Bifidobacterium breve (B. breve). A mouse hard palate mucosal defect model was used to investigate the influence of sunitinib and/or zoledronate on wound healing. The volume and density of the bone under the mucosal defect were assessed by micro-computed tomography (micro-CT). Inflammatory factors were detected by protein microarray analysis and enzyme-linked immunosorbent assay (ELISA). The senescence and biological functions were tested in oral mucosal stem cells (OMSCs) treated with sunitinib. Ligated loop experiments were used to investigate the effect of oral B. breve. Neutralizing antibody for interleukin-10 (IL-10) was used to prove the critical role of IL-10 in the pro-healing process derived from B. breve. Results showed that sunitinib caused oral mucosal wound healing impairment in mice. In vitro, sunitinib induced cellular senescence in OMSCs and affected biological functions such as proliferation, migration, and differentiation. Oral administration of B. breve reduced oral mucosal inflammation and promoted wound healing via intestinal dendritic cells (DCs)-derived IL-10. IL-10 reversed cellular senescence caused by sunitinib in OMSCs, and IL-10 neutralizing antibody blocked the ameliorative effect of B. breve on oral mucosal wound healing under sunitinib treatment conditions. In conclusion, sunitinib induces cellular senescence in OMSCs and causes oral mucosal wound healing impairment and oral administration of B. breve could improve wound healing impairment via intestinal DCs-derived IL-10.

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来源期刊
International Journal of Oral Science
International Journal of Oral Science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
31.80
自引率
1.30%
发文量
53
审稿时长
>12 weeks
期刊介绍: The International Journal of Oral Science covers various aspects of oral science and interdisciplinary fields, encompassing basic, applied, and clinical research. Topics include, but are not limited to: Oral microbiology Oral and maxillofacial oncology Cariology Oral inflammation and infection Dental stem cells and regenerative medicine Craniofacial surgery Dental material Oral biomechanics Oral, dental, and maxillofacial genetic and developmental diseases Craniofacial bone research Craniofacial-related biomaterials Temporomandibular joint disorder and osteoarthritis The journal publishes peer-reviewed Articles presenting new research results and Review Articles offering concise summaries of specific areas in oral science.
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