Id2 表达升高导致小鼠减数分裂和精子发生缺陷

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Zhen He, Rong-Ge Yan, Qin-Bang Shang, Qi-En Yang
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引用次数: 0

摘要

DNA结合抑制蛋白(ID)主要通过与E蛋白相互作用来抑制基因表达和调节细胞命运决定。所有四种ID蛋白(ID1-4)都存在于睾丸中,其中ID4在精原干细胞命运决定中起着特别重要的作用。一些证据表明,ID 蛋白参与了减数分裂过程;然而,尚未进行功能实验来验证这一观察结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Elevated Id2 expression causes defective meiosis and spermatogenesis in mice

Background

Inhibitors of DNA binding (ID) proteins mainly inhibit gene expression and regulate cell fate decisions by interacting with E-proteins. All four ID proteins (ID1–4) are present in the testis, and ID4 has a particularly important role in spermatogonial stem cell fate determination. Several lines of evidence indicate that ID proteins are involved in meiosis; however, functional experiments have not been conducted to validate this observation.

Results

In this study, we report that ID2 is enriched in spermatocytes and that forced ID2 expression in germ cells causes defects in spermatogenesis. A detailed analysis demonstrated that Id2 overexpression (Id2 OE) decreased the total number of spermatogonia and changed the dynamics of meiosis progression. Specifically, spermatocytes were enriched in the zygotene stage, and the proportion of pachytene spermatocytes was significantly decreased, indicating defects in the zygotene–pachytene transition. The number of MLH1-positive foci per cell was decreased in pachytene spermatocytes from Id2 OE testes, suggesting abnormalities in recombination. Transcriptome analysis revealed that forced Id2 expression changed the expression of a list of genes mainly associated with meiosis and spermatid development.

Conclusions

ID2 protein is expressed in spermatocytes, and its genetic ablation in the germline does not affect spermatogenesis, likely due to genetic compensation of its family members. However, forced Id2 expression changes meiosis progression and causes defects in spermiogenesis. These data provide important evidence that ID proteins play pivotal roles in male meiosis and spermatid development.

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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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