E. E. Kuvaeva, D. A. Kulikova, O. B. Simonova, I. B. Mertsalov
{"title":"黑腹果蝇D4家族齿菊酯蛋白特异性定位研究","authors":"E. E. Kuvaeva, D. A. Kulikova, O. B. Simonova, I. B. Mertsalov","doi":"10.1134/s1062360422020072","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In this work, the expression pattern of the TTH protein, which is related to the D4 protein family, was studied at the larval stage of <i>Drosophila melanogaster</i> development for the first time<i>.</i> For this purpose, polyclonal antibodies to the TTH protein and a <i>Drosophila</i> line of flies transformed with a genetic construct containing a modified <i>tth</i> locus for the expression of the GFP-tagged TTH::GFP protein have been obtained. Analysis of the expression patterns of native and labeled proteins showed the localization of TTH in the nervous system, specialized endocrine glands, and salivary glands of larvae, as well as in the structures that form the innervation of the eyes of an adult insect. It has been suggested that the <i>tth</i> gene is involved in the development of the nervous and visual systems, as well as in the functioning of organs responsible for hormonal regulation and digestion.</p>","PeriodicalId":21434,"journal":{"name":"Russian Journal of Developmental Biology","volume":"40 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Studying the Specific Localization of Toothrin Protein from Related D4 Family in Drosophila melanogaster\",\"authors\":\"E. E. Kuvaeva, D. A. Kulikova, O. B. Simonova, I. B. Mertsalov\",\"doi\":\"10.1134/s1062360422020072\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Abstract</h3><p>In this work, the expression pattern of the TTH protein, which is related to the D4 protein family, was studied at the larval stage of <i>Drosophila melanogaster</i> development for the first time<i>.</i> For this purpose, polyclonal antibodies to the TTH protein and a <i>Drosophila</i> line of flies transformed with a genetic construct containing a modified <i>tth</i> locus for the expression of the GFP-tagged TTH::GFP protein have been obtained. Analysis of the expression patterns of native and labeled proteins showed the localization of TTH in the nervous system, specialized endocrine glands, and salivary glands of larvae, as well as in the structures that form the innervation of the eyes of an adult insect. It has been suggested that the <i>tth</i> gene is involved in the development of the nervous and visual systems, as well as in the functioning of organs responsible for hormonal regulation and digestion.</p>\",\"PeriodicalId\":21434,\"journal\":{\"name\":\"Russian Journal of Developmental Biology\",\"volume\":\"40 1\",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2022-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Russian Journal of Developmental Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s1062360422020072\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Developmental Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1062360422020072","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Studying the Specific Localization of Toothrin Protein from Related D4 Family in Drosophila melanogaster
Abstract
In this work, the expression pattern of the TTH protein, which is related to the D4 protein family, was studied at the larval stage of Drosophila melanogaster development for the first time. For this purpose, polyclonal antibodies to the TTH protein and a Drosophila line of flies transformed with a genetic construct containing a modified tth locus for the expression of the GFP-tagged TTH::GFP protein have been obtained. Analysis of the expression patterns of native and labeled proteins showed the localization of TTH in the nervous system, specialized endocrine glands, and salivary glands of larvae, as well as in the structures that form the innervation of the eyes of an adult insect. It has been suggested that the tth gene is involved in the development of the nervous and visual systems, as well as in the functioning of organs responsible for hormonal regulation and digestion.
期刊介绍：
Russian Journal of Developmental Biology is a journal that publishes reviews and experimental articles dealing with various aspects of developmental biology. It presents fundamental and applied research on development, regeneration, and carcinogenesis at the molecular, cellular, and organismic levels.
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