蒙大拿甲基单胞菌(Methylomonas montana sp. nov.),第一个从山河沉积物中分离的甲基单胞菌属的无色素甲烷化菌

IF 1.3 4区 生物学 Q4 MICROBIOLOGY
R. Z. Suleimanov, E. N. Tikhonova, I. Y. Oshkin, O. V. Danilova, S. N. Dedysh
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引用次数: 1

摘要

甲基单胞菌属的好氧甲烷营养细菌栖息于广泛的栖息地,包括淡水水体、河流沉积物、湿地、稻田、垃圾填埋场覆盖土壤和水态土壤。目前所描述的这一属的大多数物种以中性粒细胞为代表,运动,生长在甲烷杆状细菌上,其色素从黄色到粉红色和红色不等。本研究报道了这些细菌的一种新的无色素分离株MW1T的特征,该菌株来自俄罗斯克拉斯诺达尔边疆区Khosta山河的沉积物。菌株MW1T在甲烷和甲醇上生长,温度范围为8-37℃(最适25-30℃),温度范围为5.5-7.5(最适6.3-7.0)。菌株MW1T的16S rRNA基因序列与已有甲基单胞菌的相似度为95.48 ~ 98.47%。与菌株MW1T最接近的系统发育亲缘关系是易北河沉积物中分离的M. fluvii EbBT。菌株MW1T全基因组序列大小为4.6 Mb,包含3个rRNA操纵子,编码颗粒甲烷单加氧酶基因簇pmoCAB等约4200个蛋白编码基因。可溶性甲烷单加氧酶在基因组中未被编码。G+C DNA含量为52.4%。菌株MW1T基因组与早期甲基单胞菌属代表基因组的平均核苷酸同源性为79.4-82.1%。我们建议将该分离物归类为甲基单胞菌属的一个新种,M. montana sp. nov.菌株MW1T (=VKM 3737T = UQM 41536T)是该新种的型菌株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Methylomonas montana sp. nov., the First Nonpigmented Methanotroph of the Genus Methylomonas, Isolated from Mountain River Sediments

Methylomonas montana sp. nov., the First Nonpigmented Methanotroph of the Genus Methylomonas, Isolated from Mountain River Sediments

Abstract

Aerobic methanotrophic bacteria of the genus Methylomonas inhabit a wide spectrum of habitats, including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, nonpigmented isolate of these bacteria, strain MW1T, which was obtained from sediments of the mountain river Khosta, Krasnodar krai, Russia. Strain MW1T grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рH 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1T displayed 95.48–98.47% similarity to those in earlier described Methylomonas species. The closest taxonomically characterized phylogenetic relative of strain MW1T was M. fluvii EbBT, isolated from the river Elbe sediments. Complete genome sequence of strain MW1T was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster pmoCAB coding for particulate methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1T and the genomes of earlier described representatives of the genus Methylomonas was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus Methylomonas, M. montana sp. nov. Strain MW1T (=VKM 3737T = UQM 41536T) is the type strain of the newly proposed species.

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来源期刊
Microbiology
Microbiology 生物-微生物学
CiteScore
2.40
自引率
13.30%
发文量
60
审稿时长
6-12 weeks
期刊介绍: Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.
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