R. Z. Suleimanov, E. N. Tikhonova, I. Y. Oshkin, O. V. Danilova, S. N. Dedysh
{"title":"蒙大拿甲基单胞菌(Methylomonas montana sp. nov.),第一个从山河沉积物中分离的甲基单胞菌属的无色素甲烷化菌","authors":"R. Z. Suleimanov, E. N. Tikhonova, I. Y. Oshkin, O. V. Danilova, S. N. Dedysh","doi":"10.1134/s0026261723602324","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\n<b>Abstract</b>—</h3><p>Aerobic methanotrophic bacteria of the genus <i>Methylomonas</i> inhabit a wide spectrum of habitats, including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, nonpigmented isolate of these bacteria, strain MW1<sup>T</sup>, which was obtained from sediments of the mountain river Khosta, Krasnodar krai, Russia. Strain MW1<sup>T</sup> grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рH 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1<sup>T</sup> displayed 95.48–98.47% similarity to those in earlier described <i>Methylomonas</i> species. The closest taxonomically characterized phylogenetic relative of strain MW1<sup>T</sup> was <i>M. fluvii</i> EbB<sup>T</sup>, isolated from the river Elbe sediments. Complete genome sequence of strain MW1<sup>T</sup> was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster <i>pmoCAB</i> coding for particulate methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1<sup>T</sup> and the genomes of earlier described representatives of the genus <i>Methylomonas</i> was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus <i>Methylomonas</i>, <i>M. montana</i> sp. nov. Strain MW1<sup>T</sup> (=VKM 3737<sup>T</sup> = UQM 41536<sup>T</sup>) is the type strain of the newly proposed species.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"74 6","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Methylomonas montana sp. nov., the First Nonpigmented Methanotroph of the Genus Methylomonas, Isolated from Mountain River Sediments\",\"authors\":\"R. Z. Suleimanov, E. N. Tikhonova, I. Y. Oshkin, O. V. Danilova, S. N. Dedysh\",\"doi\":\"10.1134/s0026261723602324\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">\\n<b>Abstract</b>—</h3><p>Aerobic methanotrophic bacteria of the genus <i>Methylomonas</i> inhabit a wide spectrum of habitats, including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, nonpigmented isolate of these bacteria, strain MW1<sup>T</sup>, which was obtained from sediments of the mountain river Khosta, Krasnodar krai, Russia. Strain MW1<sup>T</sup> grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рH 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1<sup>T</sup> displayed 95.48–98.47% similarity to those in earlier described <i>Methylomonas</i> species. The closest taxonomically characterized phylogenetic relative of strain MW1<sup>T</sup> was <i>M. fluvii</i> EbB<sup>T</sup>, isolated from the river Elbe sediments. Complete genome sequence of strain MW1<sup>T</sup> was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster <i>pmoCAB</i> coding for particulate methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1<sup>T</sup> and the genomes of earlier described representatives of the genus <i>Methylomonas</i> was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus <i>Methylomonas</i>, <i>M. montana</i> sp. nov. Strain MW1<sup>T</sup> (=VKM 3737<sup>T</sup> = UQM 41536<sup>T</sup>) is the type strain of the newly proposed species.</p>\",\"PeriodicalId\":18514,\"journal\":{\"name\":\"Microbiology\",\"volume\":\"74 6\",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-11-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s0026261723602324\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723602324","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Methylomonas montana sp. nov., the First Nonpigmented Methanotroph of the Genus Methylomonas, Isolated from Mountain River Sediments
Abstract—
Aerobic methanotrophic bacteria of the genus Methylomonas inhabit a wide spectrum of habitats, including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, nonpigmented isolate of these bacteria, strain MW1T, which was obtained from sediments of the mountain river Khosta, Krasnodar krai, Russia. Strain MW1T grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рH 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1T displayed 95.48–98.47% similarity to those in earlier described Methylomonas species. The closest taxonomically characterized phylogenetic relative of strain MW1T was M. fluvii EbBT, isolated from the river Elbe sediments. Complete genome sequence of strain MW1T was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster pmoCAB coding for particulate methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1T and the genomes of earlier described representatives of the genus Methylomonas was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus Methylomonas, M. montana sp. nov. Strain MW1T (=VKM 3737T = UQM 41536T) is the type strain of the newly proposed species.
期刊介绍:
Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.