用集合统计分析改进干涉散射显微镜

IF 1.7 4区 化学
Minsu Lee, Seok-Cheol Hong, Minhaeng Cho
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引用次数: 0

摘要

干涉散射(iSCAT)显微镜,无标签高速(高达每秒1000帧)成像和跟踪技术,已被证明是一种多功能工具,通过测量纳米颗粒和生物分子的质量和3D位置,以及可视化复杂细胞环境中纳米级事件的实时动态。然而,iSCAT信号的量化在实际中并没有直接定义。我们深入研究了与iSCAT信号处理相关的几个问题:易出错的后处理程序和在iSCAT对比惯例中缺乏统计可靠性。在简要介绍了相关光谱学的概念和原理之后,我们在这里讨论了一种可选的集合(更高数量密度的散射体)统计分析方法,该方法可用于从波动的iSCAT信号中提取散射粒子的动态信息。最后,我们将对时间相关iSCAT技术的相关方法进行展望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Upgrading interferometric scattering microscopy with ensemble statistical analysis

Upgrading interferometric scattering microscopy with ensemble statistical analysis

Upgrading interferometric scattering microscopy with ensemble statistical analysis

Interferometric scattering (iSCAT) microscopy, label-free high-speed (up to ~1000 frames per second) imaging and tracking technique, has proven to be a versatile tool by measuring the mass and 3D position of nanoparticles and biomolecules as well as visualizing real-time dynamics of nanoscale events in complex cellular environments. However, the quantification of iSCAT signals has not been straightforwardly defined in practical terms. We delve into several issues associated with signal processing in iSCAT: error-prone post-processing routine and lack of statistical reliability in the convention of iSCAT contrast. After providing a brief account of concepts and principles of correlation spectroscopy, we here discuss an alternative ensemble (higher number density of scatterers) statistical analysis that can be used to extract the dynamic information of scattering particles from fluctuating iSCAT signals. Finally, our perspective on the correlation approach toward time-correlated iSCAT technique will be presented.

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来源期刊
Bulletin of the Korean Chemical Society
Bulletin of the Korean Chemical Society Chemistry-General Chemistry
自引率
23.50%
发文量
182
期刊介绍: The Bulletin of the Korean Chemical Society is an official research journal of the Korean Chemical Society. It was founded in 1980 and reaches out to the chemical community worldwide. It is strictly peer-reviewed and welcomes Accounts, Communications, Articles, and Notes written in English. The scope of the journal covers all major areas of chemistry: analytical chemistry, electrochemistry, industrial chemistry, inorganic chemistry, life-science chemistry, macromolecular chemistry, organic synthesis, non-synthetic organic chemistry, physical chemistry, and materials chemistry.
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