An “organic-inorganic” hybrid multilayer film for comprehensive support in soft and hard tissue regeneration after osteosarcoma resection

Three major complications often occur after osteosarcoma resection: large bone defects, infectious wounds, and tumor recurrence. In addition to conventional internal fixation and auto- or allografts, multifunctional supportive treatments are needed for limb reconstruction after tumor removal. With inspiration from the "organic-inorganic" hybrid concept, we developed a freestanding polyelectrolyte membrane (PEM) using a layer-by-layer (LBL) deposition of quaternized chitosan (QCS) and hyaluronic acid (HA), and with copper-doped laponite (CuLAP) intercalation. The CuLAP demonstrated photothermal conversion capabilities under near-infrared (NIR) light irradiation, and displayed glutathione (GSH)-depleted Fenton-like catalytic activity. The further engineered PEM possesses a "brick and mortar" structure with enhanced surface roughness and stiffness. The fusion of CuLAP-mediated GSH-depleted chemodynamic treatment (CDT) and moderate photothermal therapy (PTT) facilitated tumor ablation and bactericidal effects. Moreover, the continuous release of copper ions and silicates aided angiogenesis and osteogenesis, supporting the regeneration of both soft (skin) and hard (bone) tissues. This all-in-one platform offers a promising clinical tool for assisting tissue reconstruction after osteosarcoma resection.