Jean Carlo Baudraz de Paula, Hugo Roldi Guariz, Kauê Alexandre Monteiro de Moraes, Gabriel Danilo Shimizu, Ricardo Tadeu de Faria, Halley Caixeta de Oliveira, Leonardo Fernandes Fraceto, Anderson Espirito Santo Pereira
{"title":"巴西原生种子萌发增强:凤梨科Dyckia sp.中游离赤霉素酸与纳米包封赤霉素酸的比较分析","authors":"Jean Carlo Baudraz de Paula, Hugo Roldi Guariz, Kauê Alexandre Monteiro de Moraes, Gabriel Danilo Shimizu, Ricardo Tadeu de Faria, Halley Caixeta de Oliveira, Leonardo Fernandes Fraceto, Anderson Espirito Santo Pereira","doi":"10.2478/fhort-2023-0029","DOIUrl":null,"url":null,"abstract":"Brazil is home to a great diversity of species of the genus <jats:italic>Dyckia.</jats:italic> However, many of these species are threatened due to habitat destruction and predatory exploitation. An alternative to conserving these plants is sexual propagation using plant regulators to stimulate germination. Gibberellic acid (GA<jats:sub>3</jats:sub>) is an effective regulator in this process, but its instability and ease of degradation pose challenges. Therefore, nanoencapsulation of GA<jats:sub>3</jats:sub> could be used to protect the molecule and allow controlled release. In this study, the effects of different doses of GA<jats:sub>3</jats:sub> were evaluated on the germination of four species: <jats:italic>D. cabrerae, D. dusenii, D. pottiorum</jats:italic> and <jats:italic>D. walteriana.</jats:italic> The first stage consisted of soaking the seeds in different concentrations of GA<jats:sub>3</jats:sub>, in which the species <jats:italic>D. dusenii</jats:italic> and <jats:italic>D. walteriana</jats:italic> showed significant responses to GA<jats:sub>3</jats:sub>, with an increase from 35% to more than 60% germination. However, the species <jats:italic>D. cabrerae</jats:italic> and <jats:italic>D. pottiorum</jats:italic> responded positively to GA<jats:sub>3</jats:sub> only in vegetative growth parameters. In the second stage, the use of nanoparticles of alginate/chitosan (NP ALG/CS) and chitosan/tripolyphosphate (NP CS/TPP) containing GA<jats:sub>3</jats:sub> was compared with free GA<jats:sub>3</jats:sub> and with NPs without GA<jats:sub>3</jats:sub>. It was verified that the use of nanoencapsulated GA<jats:sub>3</jats:sub> resulted in a more efficient germination response in <jats:italic>D. walteriana</jats:italic> seeds, using smaller doses of the regulator (between 0.75 mg · L<jats:sup>−1</jats:sup> and 1.0 mg · L<jats:sup>−1</jats:sup>), mainly with the ALG/ CS NPs. Therefore, the use of GA<jats:sub>3</jats:sub> is recommended for <jats:italic>D. dusenii</jats:italic> and <jats:italic>D. walteriana</jats:italic>, and for the latter, nanoparticles containing ALG/CS-GA<jats:sub>3</jats:sub> allow a reduction in the required dose.","PeriodicalId":12277,"journal":{"name":"Folia Horticulturae","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced germination of seeds native to Brazil: A comparative analysis between free and nanoencapsulated gibberellic acid in Dyckia sp. (Bromeliaceae)\",\"authors\":\"Jean Carlo Baudraz de Paula, Hugo Roldi Guariz, Kauê Alexandre Monteiro de Moraes, Gabriel Danilo Shimizu, Ricardo Tadeu de Faria, Halley Caixeta de Oliveira, Leonardo Fernandes Fraceto, Anderson Espirito Santo Pereira\",\"doi\":\"10.2478/fhort-2023-0029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Brazil is home to a great diversity of species of the genus <jats:italic>Dyckia.</jats:italic> However, many of these species are threatened due to habitat destruction and predatory exploitation. An alternative to conserving these plants is sexual propagation using plant regulators to stimulate germination. Gibberellic acid (GA<jats:sub>3</jats:sub>) is an effective regulator in this process, but its instability and ease of degradation pose challenges. Therefore, nanoencapsulation of GA<jats:sub>3</jats:sub> could be used to protect the molecule and allow controlled release. In this study, the effects of different doses of GA<jats:sub>3</jats:sub> were evaluated on the germination of four species: <jats:italic>D. cabrerae, D. dusenii, D. pottiorum</jats:italic> and <jats:italic>D. walteriana.</jats:italic> The first stage consisted of soaking the seeds in different concentrations of GA<jats:sub>3</jats:sub>, in which the species <jats:italic>D. dusenii</jats:italic> and <jats:italic>D. walteriana</jats:italic> showed significant responses to GA<jats:sub>3</jats:sub>, with an increase from 35% to more than 60% germination. However, the species <jats:italic>D. cabrerae</jats:italic> and <jats:italic>D. pottiorum</jats:italic> responded positively to GA<jats:sub>3</jats:sub> only in vegetative growth parameters. In the second stage, the use of nanoparticles of alginate/chitosan (NP ALG/CS) and chitosan/tripolyphosphate (NP CS/TPP) containing GA<jats:sub>3</jats:sub> was compared with free GA<jats:sub>3</jats:sub> and with NPs without GA<jats:sub>3</jats:sub>. It was verified that the use of nanoencapsulated GA<jats:sub>3</jats:sub> resulted in a more efficient germination response in <jats:italic>D. walteriana</jats:italic> seeds, using smaller doses of the regulator (between 0.75 mg · L<jats:sup>−1</jats:sup> and 1.0 mg · L<jats:sup>−1</jats:sup>), mainly with the ALG/ CS NPs. Therefore, the use of GA<jats:sub>3</jats:sub> is recommended for <jats:italic>D. dusenii</jats:italic> and <jats:italic>D. walteriana</jats:italic>, and for the latter, nanoparticles containing ALG/CS-GA<jats:sub>3</jats:sub> allow a reduction in the required dose.\",\"PeriodicalId\":12277,\"journal\":{\"name\":\"Folia Horticulturae\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-11-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/fhort-2023-0029\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/fhort-2023-0029","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
Enhanced germination of seeds native to Brazil: A comparative analysis between free and nanoencapsulated gibberellic acid in Dyckia sp. (Bromeliaceae)
Brazil is home to a great diversity of species of the genus Dyckia. However, many of these species are threatened due to habitat destruction and predatory exploitation. An alternative to conserving these plants is sexual propagation using plant regulators to stimulate germination. Gibberellic acid (GA3) is an effective regulator in this process, but its instability and ease of degradation pose challenges. Therefore, nanoencapsulation of GA3 could be used to protect the molecule and allow controlled release. In this study, the effects of different doses of GA3 were evaluated on the germination of four species: D. cabrerae, D. dusenii, D. pottiorum and D. walteriana. The first stage consisted of soaking the seeds in different concentrations of GA3, in which the species D. dusenii and D. walteriana showed significant responses to GA3, with an increase from 35% to more than 60% germination. However, the species D. cabrerae and D. pottiorum responded positively to GA3 only in vegetative growth parameters. In the second stage, the use of nanoparticles of alginate/chitosan (NP ALG/CS) and chitosan/tripolyphosphate (NP CS/TPP) containing GA3 was compared with free GA3 and with NPs without GA3. It was verified that the use of nanoencapsulated GA3 resulted in a more efficient germination response in D. walteriana seeds, using smaller doses of the regulator (between 0.75 mg · L−1 and 1.0 mg · L−1), mainly with the ALG/ CS NPs. Therefore, the use of GA3 is recommended for D. dusenii and D. walteriana, and for the latter, nanoparticles containing ALG/CS-GA3 allow a reduction in the required dose.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.