脱细胞小肠支架:修复肠穿孔的潜在异种移植。

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Kishor Tardalkar, Sonal Patil, Leena Chaudhari, Jeevitaa Kshersagar, Mrunal Damle, Akshay Kawale, Nilesh Bhamare, Vaishnavi Desai, Narayani Pathak, Vaishali Gaikwad, Meghnad G Joshi
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引用次数: 0

摘要

小肠穿孔是一种严重的病症,需要立即就医。传统的治疗方法是先切除小肠,再进行吻合术,但会出现小肠综合征(SBS)、吻合口漏和瘘管形成等并发症。本文展示了一种新策略,即利用异种脱细胞山羊小肠作为肠穿孔病例的小肠再生补片。山羊小肠支架经过十二烷基硫酸钠脱细胞处理,显示了一致、快速和有效的脱细胞效果。脱细胞过程中细胞外基质降解最少,同时保持了肠道结构。将脱细胞山羊小肠支架(DGSIS)植入绒毛膜(CAM)后,CAM 区域的血管生成没有明显的损失,因此可以对 DGSIS 进行体内生物相容性评估。然后,将 DGSIS 作为补片异种移植到小肠穿孔大鼠模型上。移植后 30 天,使用钡盐作为对比剂的胃肠道 X 射线成像显示小肠无渗漏或阻塞。组织学、扫描电子显微镜和免疫组化有助于分析宿主细胞在异种补片上的移植情况。异种补片表达了高水平的E-粘连蛋白、α-平滑肌肌动蛋白(α-SMA)、Occludin、Zonnula occluden(ZO-1)、Ki 67和Na+/K+-ATP酶。异种补丁随后被重新细胞化并融入宿主体内,而不会引起炎症反应。结果,脱细胞山羊小肠被用作异种移植物,可用于穿孔小肠的再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decellularized small intestine scaffolds: a potential xenograft for restoration of intestinal perforation.

Small intestine perforation is a serious medical condition that requires immediate medical attention. The traditional course of treatment entails resection followed by anastomosis; however, it has complications such as small bowel syndrome (SBS), anastomotic leakage, and fistula formation. Here, a novel strategy is demonstrated, that utilizes the xenogeneic, decellularized goat small intestine as a patch for small intestine regeneration in cases of intestinal perforation. The goat small intestine scaffold underwent sodium dodecyl sulfate decellularization, which revealed consistent, quick, and effective decellularization. Decellularization contributed the least amount of extracellular matrix degradation while maintaining the intestinal architecture. By implanting the decellularized goat small intestine scaffolds (DGSIS) on the chorioallantoic membrane (CAM), no discernible loss of angiogenesis was seen in the CAM region, and this enabled the DGSIS to be evaluated for biocompatibility in ovo. The DGSIS was then xeno-transplanted as a patch on a small intestine perforation rat model. After 30 days post transplant, barium salt used as contrast gastrointestinal X-ray imaging revealed no leakage or obstruction in the small intestine. Histology, scanning electron microscopy, and immunohistochemistry assisted in analyzing the engraftment of host cells into the xeno patch. The xeno-patch expressed high levels of E-cadherin, α-smooth muscle actin (α-SMA), Occludin, Zonnula occluden (ZO-1), Ki 67, and Na+/K+-ATPase. The xeno-patch was consequently recellularized and incorporated into the host without causing an inflammatory reaction. As an outcome, decellularized goat small intestine was employed as a xenograft and could be suitable for regeneration of the perforated small intestine.

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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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